This pull request aims to fine-tune the Dengue virus Denv (`dengue/denv) datasets for accurate genotype-level assignment. Similar to a previous PR ([https://github.com/nextstrain/dengue/pull/58](https://github.com/nextstrain/dengue/pull/58)) for the Dengue virus All (dengue/all`) dataset, it addresses the issue of cross-serotype samples being falsely assigned to genotypes within a specific serotype. For example, when querying all samples against a DENV4 dataset, there were false-positive "DENV4II" genotype calls.
To resolve this issue, the following actions were taken, inspired by a suggestion from @rneher in a Slack channel:
Adding a reconstructed root for each tree
Adding the "dengue/all" reconstructed root as an outgroup for each tree
Split the clades_genotype.tsv file by serotype
Remove further false positives in the DENV2 genotype-level calls by adding the DENV1, DENV3, and DENV4 roots as unassigned outgroups.
Description of proposed changes
This pull request aims to fine-tune the Dengue virus Denv (`dengue/denv
) datasets for accurate genotype-level assignment. Similar to a previous PR ([https://github.com/nextstrain/dengue/pull/58](https://github.com/nextstrain/dengue/pull/58)) for the Dengue virus All (
dengue/all`) dataset, it addresses the issue of cross-serotype samples being falsely assigned to genotypes within a specific serotype. For example, when querying all samples against a DENV4 dataset, there were false-positive "DENV4II" genotype calls.To resolve this issue, the following actions were taken, inspired by a suggestion from @rneher in a Slack channel:
clades_genotype.tsv
file by serotypeResults
The resulting trees with minimized cross-serotype false-positive genotype-level assignments are documented in https://github.com/nextstrain/dengue/issues/69
Related issue(s)
Checklist
The dataset has been pushed to PR https://github.com/nextstrain/nextclade_data/pull/203 and is available for testing at the links in the PR comment https://github.com/nextstrain/nextclade_data/pull/203#issuecomment-2130070919