The following samples had too few reads (<1) after quality filtering with DADA2:
...
Please check whether the correct primer sequences for trimming were supplied. Ignore that samples using `--ignore_failed_filtering` or adjust the threshold with `--min_read_counts`.
but the second part is misleading, the primer sequences for trimming are here not interesting, but the truncation cutoffs, i.e. increase --trunc_qmin/--trunc_rmin or lower --trunclenf/--trunclenr, or the error filtering, i.e. increase --max_ee.
d4straub
commented
2 weeks ago
Description of the bug
The error massage says:
but the second part is misleading, the primer sequences for trimming are here not interesting, but the truncation cutoffs, i.e. increase --trunc_qmin/--trunc_rmin or lower --trunclenf/--trunclenr, or the error filtering, i.e. increase --max_ee.
Command used and terminal output
No response
Relevant files
No response
System information
No response