nf-core / ampliseq

Amplicon sequencing analysis workflow using DADA2 and QIIME2
https://nf-co.re/ampliseq
MIT License
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Add param --quality_type #801

Open d4straub opened 6 days ago

d4straub commented 6 days ago

The parameter --quality_type is added that allows specifying the quality encoding instead of relying on automatic inference thereof. Unfortunately dada() seems not to be able to use quality type information, therefore the data needs to be read by derepFastq() first. That is a disadvantage for computational resources, so its only used when necessary, i.e. when not --quality_type "Auto".

Closes https://github.com/nf-core/ampliseq/issues/773

This is still a draft PR, because (1) I didnt test it on actual data and (2) test data would be great.

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