Closed orzechoj closed 5 years ago
I'm getting this error when I'm running ChIP-Seq Best Practice v1.0 with nextflow v 0.31.1, on Uppmax/ bianca.
ERROR ~ Error executing process > 'chippeakanno (ALBB13_137_H3K27me3_peaks)' Caused by: Process `chippeakanno (ALBB13_137_H3K27me3_peaks)` terminated with an error exit status (1) Command executed: post_peak_calling_processing.r /usr/local/lib/R/library /home/jacke/bin/chipseq/blacklists/hg19-blacklist.bed genes.gtf <a lot of data sets here..> Command exit status: 1 Command output: (empty) Command error: parLapplyLB, parRapply, parSapply, parSapplyLB The following objects are masked from ‘package:stats’: IQR, mad, sd, var, xtabs The following objects are masked from ‘package:base’: anyDuplicated, append, as.data.frame, cbind, colMeans, colnames, colSums, do.call, duplicated, eval, evalq, Filter, Find, get, grep, grepl, intersect, is.unsorted, lapply, lengths, Map, mapply, match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, Position, rank, rbind, Reduce, rowMeans, rownames, rowSums, sapply, setdiff, sort, table, tapply, union, unique, unsplit, which, which.max, which.min Loading required package: S4Vectors Attaching package: ‘S4Vectors’ The following object is masked from ‘package:base’: expand.grid Loading required package: IRanges Loading required package: GenomeInfoDb Loading required package: ChIPpeakAnno Loading required package: grid Loading required package: Biostrings Loading required package: XVector Attaching package: ‘Biostrings’ The following object is masked from ‘package:base’: strsplit Loading required package: VennDiagram Loading required package: futile.logger Warning message: In read.dcf(con) : URL 'http://bioconductor.org/BiocInstaller.dcf': status was 'Couldn't resolve host name' Loading required package: rtracklayer Loading required package: doParallel Loading required package: foreach Loading required package: iterators Error in S4Vectors:::normalize_names_replacement_value(value, x) : attempt to set too many names (2) on IRanges object of length 0 Calls: annotatePeakInBatch ... names<- -> names<- -> names<- -> names<- -> <Anonymous> Execution halted
I used the following command to run:
nohup nextflow run /home/jacke/bin/chipseq \ --singleEnd \ --reads '*.fastq.gz' \ --genome GRCh37 \ --project sens2017533 \ --macsconfig 'macssetup.config' \ -resume \ -with-trace \ -with-dag flowchart.pdf \ --broad \ --blacklist_filtering \ -profile uppmax \ -with-singularity /home/jacke/bin/singularity_images/nfcore-chipseq.img &
And most, but not all, MACS 2 jobs have finished ok.
EDIT: Two of the peak files from MACS contain no peaks. That is probably what gives the error.
Closed in favour of https://github.com/nf-core/chipseq/issues/35
I'm getting this error when I'm running ChIP-Seq Best Practice v1.0 with nextflow v 0.31.1, on Uppmax/ bianca.
I used the following command to run:
And most, but not all, MACS 2 jobs have finished ok.
EDIT: Two of the peak files from MACS contain no peaks. That is probably what gives the error.