Pipeline for the identification of extrachromosomal circular DNA (ecDNA) from Circle-seq, WGS, and ATAC-seq data that were generated from cancer and other eukaryotic cells.
When using circdna with AmpliconArchitect for a already aligned BAM file, i ran into the following problem:
Access to 'SAMTOOLS_INDEX_BAM.out' is undefined since the process 'SAMTOOLS_INDEX_BAM' has not been invoked before accessing the output attribute
-- Check script '.nextflow/assets/nf-core/circdna/./workflows/circdna.nf' at line: 302 or see '.nextflow.log' file for more details
It seems that it is due to --input_format being without capital letters.
I think it would be nice it if either the --input_format is validated and clearly stated that this should be with capital letters or maybe simply ignore case.
Description of the bug
When using circdna with AmpliconArchitect for a already aligned BAM file, i ran into the following problem:
Access to 'SAMTOOLS_INDEX_BAM.out' is undefined since the process 'SAMTOOLS_INDEX_BAM' has not been invoked before accessing the output attribute
-- Check script '.nextflow/assets/nf-core/circdna/./workflows/circdna.nf' at line: 302 or see '.nextflow.log' file for more details
It seems that it is due to --input_format being without capital letters.
I think it would be nice it if either the --input_format is validated and clearly stated that this should be with capital letters or maybe simply ignore case.
By the way thanks for implementing the tool! :)
Command used and terminal output
No response
Relevant files
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System information
No response