nf-core / circrna

circRNA quantification, differential expression analysis and miRNA target prediction of RNA-Seq data
https://nf-co.re/circrna
MIT License
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ERROR ~ Invalid method invocation `call` with arguments using DCC #161

Open ZabalaAitor opened 1 month ago

ZabalaAitor commented 1 month ago

Description of the bug

Hello,

I encountered this error using DCC.

Command used and terminal output

nextflow pull nf-core/circrna

nextflow run nf-core/circrna \
    -r dev \
    -profile apptainer \
    --input /data/azabala/RNAseq_MS/samplesheet_circRNA.csv \
    --phenotype /data/azabala/RNAseq_MS/phenotype.csv \
    --outdir /scratch/azabala/circRNA_MS \
    --tool 'dcc' \
    --limitSjdbInsertNsj 1500000 \
    --max_cpus 36 \
    --max_memory 512GB \
    -w /scratch/azabala/circRNA_MS/work_circRNA_MS \
    --genome GRCh38 \
    --save_reference false \
    --gtf /data/azabala/gtf/UCSC/NCBI/hg38.ncbiRefSeq.gtf \
    -resume

Checking nf-core/circrna ...
 Already-up-to-date - revision: 009e891b31 [dev]
N E X T F L O W  ~  version 23.04.2
Launching `https://github.com/nf-core/circrna` [ridiculous_allen] DSL2 - revision: 009e891b31 [dev]
WARN: Nextflow self-contained distribution allows only core plugins -- User config plugins will be ignored: nf-validation@1.1.3
WARN: Access to undefined parameter `monochromeLogs` -- Initialise it to a default value eg. `params.monochromeLogs = some_value`

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        ___     __   __   __   ___     /,-._.--~'
  |\ | |__  __ /  ` /  \ |__) |__         }  {
  | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                        `._,._,'
  nf-core/circrna vdev-g009e891
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Core Nextflow options
  revision          : dev
  runName           : ridiculous_allen
  containerEngine   : apptainer
  launchDir         : /scratch/azabala/circRNA_MS
  workDir           : /scratch/azabala/circRNA_MS/work_circRNA_MS
  projectDir        : /home/azabala/.nextflow/assets/nf-core/circrna
  userName          : azabala
  profile           : apptainer
  configFiles       : 

Input/output options
  input             : /data/azabala/RNAseq_MS/samplesheet_circRNA.csv
  outdir            : /scratch/azabala/circRNA_MS
  phenotype         : /data/azabala/RNAseq_MS/phenotype.csv

Discovery Options
  tool              : dcc

Alignment Options
  limitSjdbInsertNsj: 1500000

Reference genome options
  save_reference    : false
  genome            : GRCh38
  fasta             : s3://ngi-igenomes/igenomes//Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa
  gtf               : /data/azabala/gtf/UCSC/NCBI/hg38.ncbiRefSeq.gtf
  mature            : s3://ngi-igenomes/igenomes//Homo_sapiens/NCBI/GRCh38/Annotation/SmallRNA/mature.fa

Max job request options
  max_cpus          : 36
  max_memory        : 512GB

ERROR ~ Invalid method invocation `call` with arguments: [HCMS09, null, /scratch/azabala/circRNA_MS/work_circRNA_MS/90/7e326a92a9973120f782db4686e781/HCMS09_mate1.Chimeric.out.junction, /scratch/azabala/circRNA_MS/work_circRNA_MS/2a/b67e65a96aacc786bb9c4068479465/HCMS09_mate2.Chimeric.out.junction] (java.util.ArrayList) on _closure14 type

 -- Check '.nextflow.log' file for details
ERROR ~ Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting

 -- Check '.nextflow.log' file for details
-[nf-core/circrna] Pipeline completed with errors-

Relevant files

No response

System information

Nextflow: 23.04.2 Hardware: HPC Executor: slurm Conatiner: Apptainer OS: Linux nf-core/circrna: dev

nictru commented 1 month ago

I am currently travelling and will only be able to look into this early next week