nf-core / crisprseq

A pipeline for the analysis of CRISPR edited data. It allows the evaluation of the quality of gene editing experiments using targeted next generation sequencing (NGS) data (`targeted`) as well as the discovery of important genes from knock-out or activation CRISPR-Cas9 screens using CRISPR pooled DNA (`screening`).
https://nf-co.re/crisprseq
MIT License
25 stars 25 forks source link

Copy original file rather than softlink #120

Closed zhouzhendiao closed 1 month ago

zhouzhendiao commented 7 months ago

Description of feature

Softlinks became invalid after nextflow clean. It's not convenient sometimes, especially when I want to backup results. Could this pipeline copy results from cache rather than just softlink?

LaurenceKuhl commented 5 months ago

Hi @zhouzhendiao this should be fixed in the next release! In the meantime you can try running it with the dev branch. Just to be sure, could you please paste the command you use to execute the pipeline? Thank you, Laurence

zhouzhendiao commented 5 months ago

Hi @zhouzhendiao this should be fixed in the next release! In the meantime you can try running it with the dev branch. Just to be sure, could you please paste the command you use to execute the pipeline? Thank you, Laurence

Hi @LaurenceKuhl , Here is command:

nohup nextflow run /local_path/nf-core-crisprseq-2.0.0/workflow \
    --analysis screening \
    --genome GRCh38 \
    --library library_file \
    --mle_design_matrix treatment_vs_control.txt \
    -profile singularity \
    --igenomes_base  local_path  \
    -offline \
    --outdir . \
    --input samplesheet.csv \
    -config user.config \
    -resume \
    --max_cpus 16 \
    --max_memory 64.GB \
    --max_time 256.h &

# clean
nextflow log | awk -F '\t' '{print $3}' | tail -4 | xargs -i nextflow clean {} -f
/bin/rm -r .nextflow* null/ work/ pipeline_info/
LaurenceKuhl commented 4 months ago

Hi we are currently planning the next release and i will get back to you to make sure everything works!

zhouzhendiao commented 4 months ago

Thanks!

LaurenceKuhl commented 3 months ago

Hi @zhouzhendiao, could you please check the documentation and try again with version 2.2.0 and let me know if it works?

LaurenceKuhl commented 1 month ago

Hi @zhouzhendiao closing this issue, feel free to reopen it in case you have any issues :)