nf-core / eager

A fully reproducible and state-of-the-art ancient DNA analysis pipeline
https://nf-co.re/eager
MIT License
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Consider adding nonpariel - 'preseq for metagenomic taxonomic profiling' #335

Closed jfy133 closed 3 years ago

jfy133 commented 4 years ago

Is your feature request related to a problem? Please describe. Given the rising increase in microbiome studies, it might be good to include an additional QC step for deeper sequencing calculations for metagenomes.

Describe the solution you'd like This can be done with nonpariel https://academic.oup.com/bioinformatics/article/30/5/629/246142 http://enve-omics.ce.gatech.edu/nonpareil/index

Advantages:

Describe alternatives you've considered

Disadvantage:

It might not be so relevent given EAGER is meant to be genome reconstruction, but as we have MALT for first step as pathogen screening, it could be useful?

It would be good to get other people's opinions on this.

jfy133 commented 3 years ago

Doesn't support gzipped reads, so this will result in large HDD usage and I would rather not on already such a large pipeline...