Open FernandoDuarteF opened 2 hours ago
Log error:
Command error:
2024-10-25 14:10:36 INFO: [hmmsearch] 4 of 34 task(s) completed
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2024-10-25 14:10:37 WARNING: BUSCO did not find any match. Make sure to check the log files if this is unexpected.
2024-10-25 14:10:37 CRITICAL: Unhandled exception occurred:
Traceback (most recent call last):
File "/usr/local/lib/python3.7/site-packages/busco/BuscoRunner.py", line 120, in run
...skipping...
File "/usr/local/lib/python3.7/site-packages/busco/analysis/BuscoAnalysis.py", line 141, in run_hmmer
self.record_results()
File "/usr/local/lib/python3.7/site-packages/busco/analysis/GenomeAnalysis.py", line 1081, in record_results
self.hmmer_runner.record_results()
File "/usr/local/lib/python3.7/site-packages/busco/busco_tools/hmmer.py", line 1210, in record_results
self._get_busco_percentages()
File "/usr/local/lib/python3.7/site-packages/busco/busco_tools/hmmer.py", line 1309, in _get_busco_percentages
self._get_stop_codon_percent_and_avg_identity()
File "/usr/local/lib/python3.7/site-packages/busco/busco_tools/hmmer.py", line 1359, in _get_stop_codon_percent_and_avg_identity
self.avg_identity = round(sum(identities) / len(identities), 2)
ZeroDivisionError: division by zero
2024-10-25 14:10:37 ERROR: division by zero
2024-10-25 14:10:37 ERROR: BUSCO analysis failed!
2024-10-25 14:10:37 ERROR: Check the logs, read the user guide (https://busco.ezlab.org/busco_userguide.html), and check the BUSCO issue board on https://gitlab.com/ezlab/busco/issues
Pipeline fails on BUSCO step when running on testdata.
The problem seems to be related to the
input_myco_tiny.csv
dataset.Choosing
bacteria_odb10
as lineage to run BUSCO fixes the issue, but we should probably have a look at other alternatives.