nf-core / mcmicro

An end-to-end processing pipeline that transforms multi-channel whole-slide images into single-cell data.
https://nf-co.re/mcmicro
MIT License
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Consider --illumination manual vs. checking for the presence of the dfp/ffp samplesheet columns #38

Open jmuhlich opened 1 month ago

jmuhlich commented 1 month ago

Description of feature

I realized that --illumination manual may be semi-redundant with including the ffp and/or dfp columns in the sample sheet. We should either add a check in PIPELINE_INITIALISATION that these two things are aligned, or rethink the interface to this feature (perhaps the presence of user-provided illumination profiles would imply illumination="manual" and the illumination param would only serve to invoke basicpy or another future tool).