nf-core / methylseq

Methylation (Bisulfite-Sequencing) analysis pipeline using Bismark or bwa-meth + MethylDackel
https://nf-co.re/methylseq
MIT License
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Additional downstream processes written in DSL1 -- UPGRADE now to DSL2 #283

Open Jani-94 opened 1 year ago

Jani-94 commented 1 year ago

Description of feature

Already forked from methylseq (written in DSL1) and added the following:

  1. additional methylation calling with cgmaptools and general statistics (plots and tables) using cgmaptools.
  2. coverage stats and other things using methylkit.
  3. Create a plot of methylation levels across the TSS using viewBS.
  4. Input a bam file and continue from there with methylation calling etc.

Will start to update code from DSL1 to DSL2.

Cloned & modified Github: https://github.com/FAANG/GSM-pipeline

ewels commented 1 year ago

Excited to see some of these improvements! I would definitely split them up rather than trying to tackle all at once. As such, it might be worth making a new issue for each.

For each addition, you'll need to:

I'd recommend starting with the simplest, once you've gone through the process once or twice it starts getting a lot easier..

Shout on Slack if you need any help with any of this!

sateeshperi commented 1 month ago

hey @Jani-94 are there any updates for what you proposed here ?