nf-core / methylseq

Methylation (Bisulfite-Sequencing) analysis pipeline using Bismark or bwa-meth + MethylDackel
https://nf-co.re/methylseq
MIT License
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Support for NuGen Ovation RRBS libraries #379

Open jma1991 opened 6 months ago

jma1991 commented 6 months ago

This pull request introduces an initial implementation for processing NuGen Ovation RRBS libraries, drawing guidance from the commands outlined in the NuMetRRBS repository. Currently, the focus is on integrating fundamental steps such as adapter trimming and diversity trimming. Although there's an optional component for deduplicating reads via molecular tags, I'm not sure how to implement this right now. Lastly, let me know if I should submit some Ovation data to the test-datasets repository.

PR checklist

jma1991 commented 6 months ago

The suggested diversity trimming looks good to me, but do we have specific test data for this?

I have some Ovation test data, shall I submit it to the test-datasets repository?

ewels commented 6 months ago

@jma1991 could you please update / rebase this PR off the latest nf-core dev branch please?