nf-core / molkart

A pipeline for processing Molecular Cartography data from Resolve Bioscience (combinatorial FISH)
https://nf-co.re/molkart
MIT License
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Replace project spots and mcquant with faster method #11

Closed FloWuenne closed 1 year ago

FloWuenne commented 1 year ago

Description of feature

Currently the PROJECT_SPOTS module consumes a large amount of RAM and is quite slow, due to the fact that spots are actually written into 2d numpy arrays and saved as a multi-channel tiff. This behaviour is not necessarily required, as quantification of spots per cell can be performed much faster and more efficient. The default method should be more lightweight and could replace PROJECT_SPOTS and MCQUANT.

FloWuenne commented 1 year ago

Implemented in #15 !