Closed vwucher closed 6 months ago
Ok, so I cannot reproduce the error, would it be ok to send me your files, or at least a summary of the content of each of them? I am talking about:
Hi,
Thanks a lot for your answer. I am not sure I can share the data, since it is patient data but I will check. Otherwise, I manage to make it run by ignoring the process:
process {
// Ignore the error
withName: VCF_COLLECT {
errorStrategy = 'ignore'
}
}
By doing so, rnafusion
can finish:
executor > local (51)
[f8/be7f05] process > NFCORE_RNAFUSION:RNAFUSION:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv) [100%] 1 of 1, cached: 1 ✔
[- ] process > NFCORE_RNAFUSION:RNAFUSION:CAT_FASTQ -
[48/be59c4] process > NFCORE_RNAFUSION:RNAFUSION:FASTQC (E14658TPOOL-POOL-CTRL-HD784-54363-LIG1H) [100%] 23 of 23, cached: 23 ✔
[e5/5a4d78] process > NFCORE_RNAFUSION:RNAFUSION:ARRIBA_WORKFLOW:STAR_FOR_ARRIBA (E15254T-23EH16378-0-RB-LIG1H) [100%] 23 of 23, cached: 23 ✔
[e9/4b325d] process > NFCORE_RNAFUSION:RNAFUSION:ARRIBA_WORKFLOW:ARRIBA (E14658TPOOL-POOL-CTRL-HD784-54363) [100%] 23 of 23, cached: 23 ✔
[49/2eb42c] process > NFCORE_RNAFUSION:RNAFUSION:STARFUSION_WORKFLOW:STAR_FOR_STARFUSION (C467T-23EH16496-3) [100%] 23 of 23, cached: 23 ✔
[ea/3a9dbf] process > NFCORE_RNAFUSION:RNAFUSION:STARFUSION_WORKFLOW:SAMTOOLS_INDEX_FOR_STARFUSION (E15258T-23EH15799-01G-LIG1H) [100%] 23 of 23, cached: 23 ✔
[d9/57b2fe] process > NFCORE_RNAFUSION:RNAFUSION:STARFUSION_WORKFLOW:STARFUSION (E15258T-23EH15799-01G-LIG1H) [100%] 23 of 23, cached: 23 ✔
[10/99dee4] process > NFCORE_RNAFUSION:RNAFUSION:FUSIONCATCHER_WORKFLOW:FUSIONCATCHER (C469T-23EH16313-0-RUBAN) [100%] 23 of 23, cached: 23 ✔
[90/1b5128] process > NFCORE_RNAFUSION:RNAFUSION:STRINGTIE_WORKFLOW:STRINGTIE_STRINGTIE (E15258T-23EH15799-01G-LIG1H) [100%] 23 of 23, cached: 23 ✔
[d4/7f9602] process > NFCORE_RNAFUSION:RNAFUSION:STRINGTIE_WORKFLOW:STRINGTIE_MERGE (23) [100%] 23 of 23, cached: 23 ✔
[39/6e81fd] process > NFCORE_RNAFUSION:RNAFUSION:FUSIONREPORT_WORKFLOW:FUSIONREPORT (E14994T-23EH11369-08H) [100%] 23 of 23, cached: 23 ✔
[d4/2d508d] process > NFCORE_RNAFUSION:RNAFUSION:FUSIONINSPECTOR_WORKFLOW:FUSIONINSPECTOR (E15258T-23EH15799-01G) [100%] 16 of 16, cached: 11 ✔
[44/25653d] process > NFCORE_RNAFUSION:RNAFUSION:FUSIONINSPECTOR_WORKFLOW:AGAT_CONVERTSPGFF2TSV (E15258T-23EH15799-01G) [100%] 16 of 16, cached: 2 ✔
[8d/c63819] process > NFCORE_RNAFUSION:RNAFUSION:FUSIONINSPECTOR_WORKFLOW:VCF_COLLECT (E15258T-23EH15799-01G) [100%] 16 of 16, failed: 7 ✔
[ac/f84e64] process > NFCORE_RNAFUSION:RNAFUSION:FUSIONINSPECTOR_WORKFLOW:ARRIBA_VISUALISATION (E15258T-23EH15799-01G) [100%] 16 of 16, cached: 2 ✔
[3c/161c72] process > NFCORE_RNAFUSION:RNAFUSION:QC_WORKFLOW:PICARD_COLLECTRNASEQMETRICS (E15258T-23EH15799-01G-LIG1H) [100%] 23 of 23, cached: 23 ✔
[46/f281d8] process > NFCORE_RNAFUSION:RNAFUSION:QC_WORKFLOW:GATK4_MARKDUPLICATES (C468T-23EH16475-1) [100%] 23 of 23, cached: 23 ✔
[67/95b0e2] process > NFCORE_RNAFUSION:RNAFUSION:QC_WORKFLOW:PICARD_COLLECTINSERTSIZEMETRICS (C468T-23EH16475-1) [100%] 23 of 23, cached: 23 ✔
[57/e1d4a4] process > NFCORE_RNAFUSION:RNAFUSION:CUSTOM_DUMPSOFTWAREVERSIONS (1) [100%] 1 of 1 ✔
[dd/7576ed] process > NFCORE_RNAFUSION:RNAFUSION:MULTIQC [100%] 1 of 1 ✔
-[nf-core/rnafusion] Pipeline completed successfully, but with errored process(es) -
-[nf-core/rnafusion] Pipeline completed successfully, but with errored process(es) -
Completed at: 31-Jan-2024 11:03:40
Duration : 19m 46s
CPU hours : 89.1 (84.1% cached, 0% failed)
Succeeded : 44
Cached : 315
Ignored : 7
Failed : 7
Hi again,
Normally it is ok to share these processed RNA-seq data. I put the four files changing their name (sample1.zip).
Thanks again for your help, Valentin
Description of the bug
Hi, I am currently trying to run
rnafusion 3.0.1
on patient samples, but it crashes during theNFCORE_RNAFUSION:RNAFUSION:FUSIONINSPECTOR_WORKFLOW:VCF_COLLECT
process. I can make it work if I use the--skip_vis
option, but I would like to have at least the visualisation ofArriba
.Since it is for diagnosis, it is targeted RNA-seq and it is also possible that for some samples there is no fusion detected, I don't know if it can be the issue.
I don't know if it possible to either fix this or skip this process only (and not the
Arriba
visualisation).Thanks in advance for your help!
Command used and terminal output
Relevant files
No response
System information
No response