nf-core / rnaseq

RNA sequencing analysis pipeline using STAR, RSEM, HISAT2 or Salmon with gene/isoform counts and extensive quality control.
https://nf-co.re/rnaseq
MIT License
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Pipeline stops running after a few stages are complete #817

Closed anikethjr closed 2 years ago

anikethjr commented 2 years ago

Description of the bug

I am trying to process a dataset that consists of about 100 fastq files using the pipeline. I am running the pipeline on a HPC which is managed using Slurm. The first few stages of the pipeline finish successfully. However, once the NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:FASTQC and NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE stages are completed, none of the other stages spawn Slurm jobs.

When I look at the .nextflow.log file, I see that the script is polling to see if the other stages are done but there seem to be no actual jobs running them. This problem is similar to https://github.com/nextflow-io/nextflow/issues/981. I would greatly appreciate your help with this!

Command used and terminal output

$ nextflow run nf-core/rnaseq -resume -c nextflow.config --input samplesheet.csv --outdir processed_data/ --genome GRCh38 -profile singularity

[0.009s][warning][os,container] Duplicate cpuset controllers detected. Picking /sys/fs/cgroup/cpuset, skipping /cgroup/cpuset.
N E X T F L O W  ~  version 22.04.0
Launching `https://github.com/nf-core/rnaseq` [lethal_nightingale] DSL2 - revision: 7106bd792b [master]

------------------------------------------------------
                                        ,--./,-.
        ___     __   __   __   ___     /,-._.--~'
  |\ | |__  __ /  ` /  \ |__) |__         }  {
  | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                        `._,._,'
  nf-core/rnaseq v3.6
------------------------------------------------------
Core Nextflow options
  revision       : master
  runName        : lethal_nightingale
  containerEngine: singularity
  launchDir      : ##
  workDir        : ##
  projectDir     : ##
  userName       : ##
  profile        : singularity
  configFiles    : ##

Input/output options
  input          : samplesheet.csv
  outdir         : processed_data/

Reference genome options
  genome         : GRCh38
  fasta          : s3://ngi-igenomes/igenomes/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa
  gtf            : s3://ngi-igenomes/igenomes/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf
  gene_bed       : s3://ngi-igenomes/igenomes/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed
  star_index     : s3://ngi-igenomes/igenomes/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/

Max job request options
  max_cpus       : 32
  max_time       : 240h

!! Only displaying parameters that differ from the pipeline defaults !!
------------------------------------------------------
If you use nf-core/rnaseq for your analysis please cite:

* The pipeline
  https://doi.org/10.5281/zenodo.1400710

* The nf-core framework
  https://doi.org/10.1038/s41587-020-0439-x

* Software dependencies
  https://github.com/nf-core/rnaseq/blob/master/CITATIONS.md
------------------------------------------------------
WARN: =============================================================================
  When using '--genome GRCh38' the assembly is from the NCBI and NOT Ensembl.
  Biotype QC will be skipped to circumvent the issue below:
  https://github.com/nf-core/rnaseq/issues/460

  If you would like to use the soft-masked Ensembl assembly instead please see:
  https://github.com/nf-core/rnaseq/issues/159#issuecomment-501184312
===================================================================================
[-        ] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:GTF_GENE_FILTER                       -
[-        ] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:GTF_GENE_FILTER                       -
[-        ] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:MAKE_TRANSCRIPTS_FASTA                -
[-        ] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:GTF_GENE_FILTER                       -
[-        ] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:MAKE_TRANSCRIPTS_FASTA                -
[24/d026f7] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES (genome.fa)      [100%] 1 of 1, cached: 1 ✔
[3c/cc0bde] process > NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)      [100%] 1 of 1, cached: 1 ✔
[-        ] process > NFCORE_RNASEQ:RNASEQ:CAT_FASTQ                                            -
[08/793219] process > NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:FASTQC (YT)               [100%] 100 of 100, cache...
[19/fc3a1b] process > NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (YT)           [100%] 100 of 100, cache...
[-        ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN                                -
[07/1c3ecb] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:GTF_GENE_FILTER (genome.fa)           [100%] 1 of 1, cached: 1 ✔
[-        ] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:MAKE_TRANSCRIPTS_FASTA                -
[24/d026f7] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES (genome.fa)      [100%] 1 of 1, cached: 1 ✔
[3c/cc0bde] process > NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)      [100%] 1 of 1, cached: 1 ✔
[-        ] process > NFCORE_RNASEQ:RNASEQ:CAT_FASTQ                                            -
[08/793219] process > NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:FASTQC (YT)               [100%] 100 of 100, cache...
[19/fc3a1b] process > NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (YT)           [100%] 100 of 100, cache...
[-        ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN                                -
[07/1c3ecb] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:GTF_GENE_FILTER (genome.fa)           [100%] 1 of 1, cached: 1 ✔
[-        ] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:MAKE_TRANSCRIPTS_FASTA                -
[24/d026f7] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES (genome.fa)      [100%] 1 of 1, cached: 1 ✔
[3c/cc0bde] process > NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)      [100%] 1 of 1, cached: 1 ✔
[-        ] process > NFCORE_RNASEQ:RNASEQ:CAT_FASTQ                                            -
[08/793219] process > NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:FASTQC (YT)               [100%] 100 of 100, cache...
[19/fc3a1b] process > NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (YT)           [100%] 100 of 100, cache...
[-        ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN                                -
[-        ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_SORT           -
[07/1c3ecb] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:GTF_GENE_FILTER (genome.fa)           [100%] 1 of 1, cached: 1 ✔
[31/c24bb6] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:MAKE_TRANSCRIPTS_FASTA (rsem/genom... [100%] 1 of 1, cached: 1 ✔
[24/d026f7] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES (genome.fa)      [100%] 1 of 1, cached: 1 ✔
[3c/cc0bde] process > NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)      [100%] 1 of 1, cached: 1 ✔
[-        ] process > NFCORE_RNASEQ:RNASEQ:CAT_FASTQ                                            -
[08/793219] process > NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:FASTQC (YT)               [100%] 100 of 100, cache...
[19/fc3a1b] process > NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (YT)           [100%] 100 of 100, cache...
[-        ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN                                -
[-        ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_SORT           -
[-        ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_INDEX          -
[-        ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:S... -
[-        ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:S... -
[-        ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:S... -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT                    -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_TX2GENE                  -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_TXIMPORT                 -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE                  -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE_LENGTH_SCALED    -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE_SCALED           -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_TRANSCRIPT            -
[-        ] process > NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_STAR_SALMON                                -
[-        ] process > NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_FAIL_MAPPED                              -
[-        ] process > NFCORE_RNASEQ:RNASEQ:PRESEQ_LCEXTRAP                                      -
[-        ] process > NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:PICARD_MARKDUPLICATES         -
[-        ] process > NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:SAMTOOLS_INDEX                -
[-        ] process > NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOL... -
[-        ] process > NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOL... -
[-        ] process > NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOL... -
[-        ] process > NFCORE_RNASEQ:RNASEQ:STRINGTIE                                            -
[-        ] process > NFCORE_RNASEQ:RNASEQ:SUBREAD_FEATURECOUNTS                                -
[-        ] process > NFCORE_RNASEQ:RNASEQ:MULTIQC_CUSTOM_BIOTYPE                               -
[-        ] process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV                                   -
[-        ] process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_FORWARD:UCSC_BEDCLIP              -
[-        ] process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_FORWARD:UCSC_BEDGRAPHTOBIGWIG     -
[-        ] process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_REVERSE:UCSC_BEDCLIP              -
[-        ] process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_REVERSE:UCSC_BEDGRAPHTOBIGWIG     -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUALIMAP_RNASEQ                                      -
[-        ] process > NFCORE_RNASEQ:RNASEQ:DUPRADAR                                             -
[-        ] process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_BAMSTAT                                  -
[-        ] process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_INNERDISTANCE                            -
[-        ] process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_INFEREXPERIMENT                          -
[-        ] process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_JUNCTIONANNOTATION                       -
[-        ] process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_JUNCTIONSATURATION                       -
[-        ] process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_READDISTRIBUTION                         -
[-        ] process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_READDUPLICATION                          -
[-        ] process > NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_STRAND_CHECK                             -
[-        ] process > NFCORE_RNASEQ:RNASEQ:CUSTOM_DUMPSOFTWAREVERSIONS                          -
[-        ] process > NFCORE_RNASEQ:RNASEQ:MULTIQC                                              -
Staging foreign file: s3://ngi-igenomes/igenomes/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa
Staging foreign file: s3://ngi-igenomes/igenomes/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa
WARN: =============================================================================
  Biotype attribute 'gene_biotype' not found in the last column of the GTF file!

  Biotype QC will be skipped to circumvent the issue below:
  https://github.com/nf-core/rnaseq/issues/460

  Amend '--featurecounts_group_type' to change this behaviour.
===================================================================================

Relevant files

Apr-30 03:51:27.473 [Task monitor] DEBUG n.processor.TaskPollingMonitor - No more task to compute -- The following nodes are still active: [process] NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (value) bound ; channel: index port 2: (value) bound ; channel: gtf port 3: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_SORT status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_INDEX status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (value) bound ; channel: fasta port 2: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (value) bound ; channel: index port 2: (value) bound ; channel: gtf port 3: (value) bound ; channel: transcript_fasta port 4: (value) bound ; channel: alignment_mode port 5: (value) bound ; channel: lib_type port 6: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_TX2GENE status=ACTIVE port 0: (value) OPEN ; channel: salmon/* port 1: (value) bound ; channel: gtf port 2: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_TXIMPORT status=ACTIVE port 0: (value) OPEN ; channel: salmon/* port 1: (value) OPEN ; channel: tx2gene port 2: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE status=ACTIVE port 0: (value) OPEN ; channel: counts port 1: (value) OPEN ; channel: tpm port 2: (value) OPEN ; channel: tx2gene port 3: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE_LENGTH_SCALED status=ACTIVE port 0: (value) OPEN ; channel: counts port 1: (value) OPEN ; channel: tpm port 2: (value) OPEN ; channel: tx2gene port 3: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE_SCALED status=ACTIVE port 0: (value) OPEN ; channel: counts port 1: (value) OPEN ; channel: tpm port 2: (value) OPEN ; channel: tx2gene port 3: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_TRANSCRIPT status=ACTIVE port 0: (value) OPEN ; channel: counts port 1: (value) OPEN ; channel: tpm port 2: (value) OPEN ; channel: tx2gene port 3: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_STAR_SALMON status=ACTIVE port 0: (value) OPEN ; channel: counts port 1: (value) bound ; channel: pca_header_multiqc port 2: (value) bound ; channel: clustering_header_multiqc port 3: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_FAIL_MAPPED status=ACTIVE port 0: (value) OPEN ; channel: tsv_data port 1: (value) bound ; channel: header port 2: (value) bound ; channel: out_prefix port 3: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:PRESEQ_LCEXTRAP status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:PICARD_MARKDUPLICATES status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:SAMTOOLS_INDEX status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (value) bound ; channel: fasta port 2: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:STRINGTIE status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (value) bound ; channel: gtf port 2: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:SUBREAD_FEATURECOUNTS status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:MULTIQC_CUSTOM_BIOTYPE status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (value) bound ; channel: header port 2: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_FORWARD:UCSC_BEDCLIP status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (value) bound ; channel: sizes port 2: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_FORWARD:UCSC_BEDGRAPHTOBIGWIG status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (value) bound ; channel: sizes port 2: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_REVERSE:UCSC_BEDCLIP status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (value) bound ; channel: sizes port 2: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_REVERSE:UCSC_BEDGRAPHTOBIGWIG status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (value) bound ; channel: sizes port 2: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUALIMAP_RNASEQ status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (value) bound ; channel: gtf port 2: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:DUPRADAR status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (value) bound ; channel: gtf port 2: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_BAMSTAT status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_INNERDISTANCE status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (value) bound ; channel: bed port 2: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_INFEREXPERIMENT status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (value) bound ; channel: bed port 2: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_JUNCTIONANNOTATION status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (value) bound ; channel: bed port 2: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_JUNCTIONSATURATION status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (value) bound ; channel: bed port 2: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_READDISTRIBUTION status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (value) bound ; channel: bed port 2: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_READDUPLICATION status=ACTIVE port 0: (queue) OPEN ; channel: - port 1: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_STRAND_CHECK status=ACTIVE port 0: (value) OPEN ; channel: tsv_data port 1: (value) bound ; channel: header port 2: (value) bound ; channel: out_prefix port 3: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:CUSTOM_DUMPSOFTWAREVERSIONS status=ACTIVE port 0: (queue) OPEN ; channel: versions port 1: (cntrl) - ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:MULTIQC status=ACTIVE port 0: (value) bound ; channel: multiqc_config port 1: (value) bound ; channel: multiqc_custom_config port 2: (value) OPEN ; channel: software_versions port 3: (queue) OPEN ; channel: workflow_summary port 4: (value) OPEN ; channel: fail_mapping_summary port 5: (value) OPEN ; channel: fail_strand_check port 6: (value) bound ; channel: fastqc/ port 7: (value) bound ; channel: trimgalore/fastqc/ port 8: (value) bound ; channel: trimgalore/ port 9: (value) bound ; channel: sortmerna/ port 10: (value) OPEN ; channel: star/ port 11: (value) bound ; channel: hisat2/ port 12: (value) bound ; channel: rsem/ port 13: (value) bound ; channel: salmon/ port 14: (value) OPEN ; channel: samtools/stats/ port 15: (value) OPEN ; channel: samtools/flagstat/ port 16: (value) OPEN ; channel: samtools/idxstats/ port 17: (value) OPEN ; channel: picard/markduplicates/ port 18: (value) OPEN ; channel: featurecounts/ port 19: (value) OPEN ; channel: deseq2/aligner/ port 20: (value) OPEN ; channel: deseq2/aligner/ port 21: (value) bound ; channel: deseq2/pseudoaligner/ port 22: (value) bound ; channel: deseq2/pseudoaligner/ port 23: (value) OPEN ; channel: preseq/ port 24: (value) OPEN ; channel: qualimap/ port 25: (value) OPEN ; channel: dupradar/ port 26: (value) OPEN ; channel: rseqc/bam_stat/ port 27: (value) OPEN ; channel: rseqc/infer_experiment/ port 28: (value) OPEN ; channel: rseqc/inner_distance/ port 29: (value) OPEN ; channel: rseqc/junction_annotation/ port 30: (value) OPEN ; channel: rseqc/junction_saturation/ port 31: (value) OPEN ; channel: rseqc/read_distribution/ port 32: (value) OPEN ; channel: rseqc/read_duplication/ port 33: (value) bound ; channel: rseqc/tin/ port 34: (cntrl) - ; channel: $

System information

Nextflow version: 22.04.0 Hardware: HPC Executor: slurm Container engine: Singularity OS: CentOS Linux Version of nf-core/rnaseq: 3.6

anikethjr commented 2 years ago

It took a few hours but it looks like the pipeline does make progress!

l0ka commented 2 years ago

I'm experiencing a similar issue. It is affecting only 2 out of 412 RNA-seq samples. I run the pipeline on a HPC, using both qsub and an interactive node. It always quit after 7 steps. I tried to wait to see if the missing steps run successfully but there's no way, unfortunately. I'm using version 3.8:

nextflow run nf-core/rnaseq \
    -r 3.8 \
    -profile singularity \
    --input ${samplesheet} \
    --outdir ${out_dir} \
    --genome GRCh38 \
    -c $cfg

Terminal output:

executor >  pbs (6), local (1)
[c8/d68284] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES (genome.fa)                   [100%] 1 of 1 ✔
[5d/d8adc8] process > NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)                   [100%] 1 of 1 ✔
[-        ] process > NFCORE_RNASEQ:RNASEQ:CAT_FASTQ                                                         -
[f4/32d79b] process > NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:FASTQC (GBM041)                        [100%] 1 of 1 ✔
[35/4d7c38] process > NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (GBM041)                    [100%] 1 of 1 ✔
[4e/eee04c] process > NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_FAIL_TRIMMED                                          [100%] 1 of 1 ✔
[-        ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN                                             -
[-        ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_SORT                        -
[-        ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_INDEX                       -
[-        ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS    -
[-        ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT -
[-        ] process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT                                 -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_TX2GENE                               -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_TXIMPORT                              -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE                               -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE_LENGTH_SCALED                 -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE_SCALED                        -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_TRANSCRIPT                         -
[-        ] process > NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_STAR_SALMON                                             -
[-        ] process > NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_FAIL_MAPPED                                           -
[-        ] process > NFCORE_RNASEQ:RNASEQ:PRESEQ_LCEXTRAP                                                   -
[-        ] process > NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:PICARD_MARKDUPLICATES                      -
[-        ] process > NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:SAMTOOLS_INDEX                             -
[-        ] process > NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS          -
[-        ] process > NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT       -
[-        ] process > NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS       -
[-        ] process > NFCORE_RNASEQ:RNASEQ:STRINGTIE_STRINGTIE                                               -
[-        ] process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV                                                -
[-        ] process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_FORWARD:UCSC_BEDCLIP                           -
[-        ] process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_FORWARD:UCSC_BEDGRAPHTOBIGWIG                  -
[-        ] process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_REVERSE:UCSC_BEDCLIP                           -
[-        ] process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_REVERSE:UCSC_BEDGRAPHTOBIGWIG                  -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUALIMAP_RNASEQ                                                   -
[-        ] process > NFCORE_RNASEQ:RNASEQ:DUPRADAR                                                          -
[-        ] process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_BAMSTAT                                               -
[-        ] process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_INNERDISTANCE                                         -
[-        ] process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_INFEREXPERIMENT                                       -
[-        ] process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_JUNCTIONANNOTATION                                    -
[-        ] process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_JUNCTIONSATURATION                                    -
[-        ] process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_READDISTRIBUTION                                      -
[-        ] process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_READDUPLICATION                                       -
[-        ] process > NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_STRAND_CHECK                                          -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_QUANT                                      -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_TX2GENE                                    -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_TXIMPORT                                   -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_GENE                                    -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_GENE_LENGTH_SCALED                      -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_GENE_SCALED                             -
[-        ] process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_TRANSCRIPT                              -
[-        ] process > NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_SALMON                                                  -
[d5/d201bf] process > NFCORE_RNASEQ:RNASEQ:CUSTOM_DUMPSOFTWAREVERSIONS (1)                                   [100%] 1 of 1 ✔
[ff/9dabab] process > NFCORE_RNASEQ:RNASEQ:MULTIQC (1)                                                       [100%] 1 of 1 ✔
-[nf-core/rnaseq] Pipeline completed successfully-
Completed at: 26-Aug-2022 10:05:46
Duration    : 12m 3s
CPU hours   : 0.2
Succeeded   : 7

Log file:

Aug-26 09:53:38.044 [main] DEBUG nextflow.cli.Launcher - $> nextflow run nf-core/rnaseq -r 3.8 -profile singularity --input /test/SAMPLE/samplesheet.csv --outdir /test/SAMPLE --genome GRCh38 -c rnaseq_GRCh38.conf
Aug-26 09:53:38.127 [main] INFO  nextflow.cli.CmdRun - N E X T F L O W  ~  version 22.04.5
Aug-26 09:53:39.444 [main] DEBUG nextflow.scm.AssetManager - Git config: /test/.nextflow/assets/nf-core/rnaseq/.git/config; branch: master; remote: origin; url: https://github.com/nf-core/rnaseq.git
Aug-26 09:53:39.462 [main] DEBUG nextflow.scm.AssetManager - Git config: /test/.nextflow/assets/nf-core/rnaseq/.git/config; branch: master; remote: origin; url: https://github.com/nf-core/rnaseq.git
Aug-26 09:53:41.267 [main] DEBUG nextflow.config.ConfigBuilder - Found config base: /test/.nextflow/assets/nf-core/rnaseq/nextflow.config
Aug-26 09:53:41.296 [main] DEBUG nextflow.config.ConfigBuilder - User config file: rnaseq_GRCh38.conf
Aug-26 09:53:41.297 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /test/.nextflow/assets/nf-core/rnaseq/nextflow.config
Aug-26 09:53:41.297 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: rnaseq_GRCh38.conf
Aug-26 09:53:41.318 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `singularity`
Aug-26 09:53:41.705 [main] DEBUG nextflow.plugin.PluginsFacade - Using Default plugins manager
Aug-26 09:53:41.724 [main] INFO  org.pf4j.DefaultPluginStatusProvider - Enabled plugins: []
Aug-26 09:53:41.725 [main] INFO  org.pf4j.DefaultPluginStatusProvider - Disabled plugins: []
Aug-26 09:53:41.728 [main] INFO  org.pf4j.DefaultPluginManager - PF4J version 3.4.1 in 'deployment' mode
Aug-26 09:53:41.737 [main] DEBUG nextflow.plugin.PluginsFacade - Using Default plugins manager
Aug-26 09:53:42.192 [main] DEBUG nextflow.plugin.PluginsFacade - Using Default plugins manager
Aug-26 09:53:42.192 [main] DEBUG nextflow.plugin.PluginsFacade - Using Default plugins manager
Aug-26 09:53:43.383 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `singularity`
Aug-26 09:53:43.454 [main] DEBUG nextflow.config.ConfigBuilder - Available config profiles: [cfc_dev, ifb_core, denbi_qbic, genotoul, alice, mjolnir_globe, uppmax, abims, nihbiowulf, nu_genomics, oist, sahmri, mpcdf, leicester, lugh, vsc_ugent, sage, cambridge, unibe_ibu, vai, podman, czbiohub_aws, jax, cheaha, ccga_med, test, google, computerome, seg_globe, sanger, pasteur, test_full, eddie, medair, azurebatch, bi, bigpurple, cedars, docker, gis, eva, utd_ganymede, charliecloud, fgcz, conda, singularity, icr_davros, munin, rosalind, prince, hasta, hebbe, cfc, utd_sysbio, uzh, debug, genouest, cbe, ebc, ccga_dx, crick, marvin, phoenix, biohpc_gen, shifter, awsbatch, uct_hpc, imperial, maestro, aws_tower, binac]
Aug-26 09:53:43.512 [main] DEBUG nextflow.cli.CmdRun - Applied DSL=2 from script declararion
Aug-26 09:53:43.513 [main] INFO  nextflow.cli.CmdRun - Launching `https://github.com/nf-core/rnaseq` [zen_lovelace] DSL2 - revision: 6995330476 [3.8]
Aug-26 09:53:43.514 [main] DEBUG nextflow.plugin.PluginsFacade - Setting up plugin manager > mode=prod; plugins-dir=/test/.nextflow/plugins; core-plugins: nf-amazon@1.7.2,nf-azure@0.13.2,nf-console@1.0.3,nf-ga4gh@1.0.3,nf-google@1.1.4,nf-sqldb@0.4.0,nf-tower@1.4.0
Aug-26 09:53:43.516 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins default=[]
Aug-26 09:53:43.529 [main] INFO  org.pf4j.DefaultPluginStatusProvider - Enabled plugins: []
Aug-26 09:53:43.530 [main] INFO  org.pf4j.DefaultPluginStatusProvider - Disabled plugins: []
Aug-26 09:53:43.531 [main] INFO  org.pf4j.DefaultPluginManager - PF4J version 3.4.1 in 'deployment' mode
Aug-26 09:53:43.543 [main] INFO  org.pf4j.AbstractPluginManager - No plugins
Aug-26 09:53:43.632 [main] DEBUG nextflow.Session - Session uuid: 1ee7c3e7-69f7-4083-8a7e-5a54b5d6d828
Aug-26 09:53:43.632 [main] DEBUG nextflow.Session - Run name: zen_lovelace
Aug-26 09:53:43.632 [main] DEBUG nextflow.Session - Executor pool size: 20
Aug-26 09:53:43.674 [main] DEBUG nextflow.cli.CmdRun - 
  Version: 22.04.5 build 5708
  Created: 15-07-2022 16:09 UTC (18:09 CEST)
  System: Linux 3.10.0-1062.4.1.el7.x86_64
  Runtime: Groovy 3.0.10 on OpenJDK 64-Bit Server VM 18.0.1+10-24
  Encoding: UTF-8 (UTF-8)
  Process: 21461@g-04-c0249 [10.45.17.42]
  CPUs: 20 - Mem: 80 GB (79.5 GB) - Swap: 16 GB (12.8 GB)
Aug-26 09:53:43.713 [main] DEBUG nextflow.Session - Work-dir: /test/SAMPLE/work [nfs]
Aug-26 09:53:43.743 [main] DEBUG nextflow.executor.ExecutorFactory - Extension executors providers=[]
Aug-26 09:53:43.759 [main] DEBUG nextflow.Session - Observer factory: DefaultObserverFactory
Aug-26 09:53:43.878 [main] DEBUG nextflow.cache.CacheFactory - Using Nextflow cache factory: nextflow.cache.DefaultCacheFactory
Aug-26 09:53:43.903 [main] DEBUG nextflow.util.CustomThreadPool - Creating default thread pool > poolSize: 21; maxThreads: 1000
Aug-26 09:53:44.046 [main] DEBUG nextflow.Session - Session start invoked
Aug-26 09:53:44.050 [main] DEBUG nextflow.trace.TraceFileObserver - Flow starting -- trace file: /test/SAMPLE/pipeline_info/execution_trace_2022-08-26_09-53-42.txt
Aug-26 09:53:44.081 [main] DEBUG nextflow.Session - Using default localLib path: /test/.nextflow/assets/nf-core/rnaseq/lib
Aug-26 09:53:44.086 [main] DEBUG nextflow.Session - Adding to the classpath library: /test/.nextflow/assets/nf-core/rnaseq/lib
Aug-26 09:53:44.087 [main] DEBUG nextflow.Session - Adding to the classpath library: /test/.nextflow/assets/nf-core/rnaseq/lib/nfcore_external_java_deps.jar
Aug-26 09:53:45.006 [main] DEBUG nextflow.script.ScriptRunner - > Launching execution
Aug-26 09:53:45.302 [main] INFO  nextflow.Nextflow - 

------------------------------------------------------
                                        ,--./,-.
        ___     __   __   __   ___     /,-._.--~'
  |\ | |__  __ /  ` /  \ |__) |__         }  {
  | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                        `._,._,'
  nf-core/rnaseq v3.8
------------------------------------------------------
Core Nextflow options
  revision              : 3.8
  runName               : zen_lovelace
  containerEngine       : singularity
  launchDir             : /test/SAMPLE
  workDir               : /test/SAMPLE/work
  projectDir            : /test/.nextflow/assets/nf-core/rnaseq
  userName              : aluloc
  profile               : singularity
  configFiles           : /test/.nextflow/assets/nf-core/rnaseq/nextflow.config, rnaseq_GRCh38.conf

Input/output options
  input                 : /test/SAMPLE/samplesheet.csv
  outdir                : /test/SAMPLE

Read filtering options
  ribo_database_manifest: /GRCh38/rRNA_db.txt

Reference genome options
  genome                : GRCh38
  fasta                 : /GRCh38/references/WholeGenomeFasta/genome.fa
  gtf                   : /GRCh38/references/genes.gtf
  gene_bed              : /GRCh38/references/genes.bed
  transcript_fasta      : /GRCh38/transcripts.fa
  star_index            : /GRCh38/STARIndex/
  salmon_index          : /GRCh38/salmon_index/
  hisat2_build_memory   : 180.GB
  gencode               : true
  igenomes_base         : /GRCh38/igenomes/Homo_sapiens/NCBI/GRCh38/
  igenomes_ignore       : true

Alignment options
  pseudo_aligner        : salmon

Process skipping options
  skip_biotype_qc       : true

Max job request options
  max_cpus              : 10

!! Only displaying parameters that differ from the pipeline defaults !!
------------------------------------------------------
If you use nf-core/rnaseq for your analysis please cite:

* The pipeline
  https://doi.org/10.5281/zenodo.1400710

* The nf-core framework
  https://doi.org/10.1038/s41587-020-0439-x

* Software dependencies
  https://github.com/nf-core/rnaseq/blob/master/CITATIONS.md
------------------------------------------------------
Aug-26 09:53:46.356 [main] WARN  nextflow.Nextflow - =============================================================================
  '--transcript_fasta' parameter has been provided.
  Make sure transcript names in this file match those in the GFF/GTF file.

  Please see:
  https://github.com/nf-core/rnaseq/issues/753
===================================================================================
Aug-26 09:53:54.458 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES
Aug-26 09:53:54.461 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:CUSTOM_GETCHROMSIZES` matches process NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES
Aug-26 09:53:54.482 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.482 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.490 [main] DEBUG nextflow.executor.Executor - [warm up] executor > pbs
Aug-26 09:53:54.501 [main] DEBUG n.processor.TaskPollingMonitor - Creating task monitor for executor 'pbs' > capacity: 100; pollInterval: 5s; dumpInterval: 5m 
Aug-26 09:53:54.510 [main] DEBUG n.executor.AbstractGridExecutor - Creating executor 'pbs' > queue-stat-interval: 1m
Aug-26 09:53:54.631 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:SAMPLESHEET_CHECK` matches process NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK
Aug-26 09:53:54.635 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.635 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.691 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNASEQ:RNASEQ:CAT_FASTQ
Aug-26 09:53:54.691 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:CAT_FASTQ` matches process NFCORE_RNASEQ:RNASEQ:CAT_FASTQ
Aug-26 09:53:54.693 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.693 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.710 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:FASTQC
Aug-26 09:53:54.711 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:FASTQC_UMITOOLS_TRIMGALORE:FASTQC` matches process NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:FASTQC
Aug-26 09:53:54.712 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.713 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.722 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_high` matches labels `process_high` for process with name NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE
Aug-26 09:53:54.723 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE` matches process NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE
Aug-26 09:53:54.726 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.726 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.754 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:MULTIQC_TSV_FAIL_TRIMMED` matches process NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_FAIL_TRIMMED
Aug-26 09:53:54.755 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: local
Aug-26 09:53:54.755 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local'
Aug-26 09:53:54.760 [main] DEBUG nextflow.executor.Executor - [warm up] executor > local
Aug-26 09:53:54.765 [main] DEBUG n.processor.LocalPollingMonitor - Creating local task monitor for executor 'local' > cpus=20; memory=80 GB; capacity=20; pollInterval=100ms; dumpInterval=5m
Aug-26 09:53:54.780 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_high` matches labels `process_high` for process with name NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN
Aug-26 09:53:54.781 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:ALIGN_STAR:STAR_ALIGN` matches process NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN
Aug-26 09:53:54.781 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN` matches process NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN
Aug-26 09:53:54.782 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.782 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.794 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_SORT
Aug-26 09:53:54.795 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:NFCORE_RNASEQ:RNASEQ:.*:BAM_SORT_SAMTOOLS:SAMTOOLS_SORT` matches process NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_SORT
Aug-26 09:53:54.796 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.796 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.802 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_INDEX
Aug-26 09:53:54.802 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:NFCORE_RNASEQ:RNASEQ:.*:BAM_SORT_SAMTOOLS:SAMTOOLS_INDEX` matches process NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_INDEX
Aug-26 09:53:54.803 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.803 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.819 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS
Aug-26 09:53:54.820 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:NFCORE_RNASEQ:RNASEQ:.*:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:.*` matches process NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS
Aug-26 09:53:54.820 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.821 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.835 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT
Aug-26 09:53:54.836 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:NFCORE_RNASEQ:RNASEQ:.*:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:.*` matches process NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT
Aug-26 09:53:54.837 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.837 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.842 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS
Aug-26 09:53:54.843 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:NFCORE_RNASEQ:RNASEQ:.*:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:.*` matches process NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS
Aug-26 09:53:54.843 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.843 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.857 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT
Aug-26 09:53:54.858 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:QUANTIFY_STAR_SALMON:SALMON_QUANT` matches process NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT
Aug-26 09:53:54.859 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.859 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.870 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_TX2GENE
Aug-26 09:53:54.872 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:QUANTIFY_STAR_SALMON:SALMON_TX2GENE` matches process NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_TX2GENE
Aug-26 09:53:54.873 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.873 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.880 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_TXIMPORT
Aug-26 09:53:54.881 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:QUANTIFY_STAR_SALMON:SALMON_TXIMPORT` matches process NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_TXIMPORT
Aug-26 09:53:54.882 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.882 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.888 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE
Aug-26 09:53:54.888 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:QUANTIFY_STAR_SALMON:SALMON_SE_.*` matches process NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE
Aug-26 09:53:54.889 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.889 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.894 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE_LENGTH_SCALED
Aug-26 09:53:54.895 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:QUANTIFY_STAR_SALMON:SALMON_SE_.*` matches process NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE_LENGTH_SCALED
Aug-26 09:53:54.895 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.895 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.899 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE_SCALED
Aug-26 09:53:54.899 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:QUANTIFY_STAR_SALMON:SALMON_SE_.*` matches process NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE_SCALED
Aug-26 09:53:54.900 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.900 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.903 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_TRANSCRIPT
Aug-26 09:53:54.903 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:QUANTIFY_STAR_SALMON:SALMON_SE_.*` matches process NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_TRANSCRIPT
Aug-26 09:53:54.904 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.904 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.910 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_STAR_SALMON
Aug-26 09:53:54.910 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:DESEQ2_QC_STAR_SALMON` matches process NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_STAR_SALMON
Aug-26 09:53:54.911 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.911 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.931 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:MULTIQC_TSV_FAIL_MAPPED` matches process NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_FAIL_MAPPED
Aug-26 09:53:54.938 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: local
Aug-26 09:53:54.938 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local'
Aug-26 09:53:54.944 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium,error_ignore` for process with name NFCORE_RNASEQ:RNASEQ:PRESEQ_LCEXTRAP
Aug-26 09:53:54.944 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:error_ignore` matches labels `process_medium,error_ignore` for process with name NFCORE_RNASEQ:RNASEQ:PRESEQ_LCEXTRAP
Aug-26 09:53:54.944 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:PRESEQ_LCEXTRAP` matches process NFCORE_RNASEQ:RNASEQ:PRESEQ_LCEXTRAP
Aug-26 09:53:54.946 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.946 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.956 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:PICARD_MARKDUPLICATES
Aug-26 09:53:54.956 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:MARK_DUPLICATES_PICARD:PICARD_MARKDUPLICATES` matches process NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:PICARD_MARKDUPLICATES
Aug-26 09:53:54.957 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.957 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.973 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:SAMTOOLS_INDEX
Aug-26 09:53:54.974 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:MARK_DUPLICATES_PICARD:SAMTOOLS_INDEX` matches process NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:SAMTOOLS_INDEX
Aug-26 09:53:54.974 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.975 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.990 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS
Aug-26 09:53:54.991 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:.*` matches process NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS
Aug-26 09:53:54.991 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.991 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:54.997 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT
Aug-26 09:53:54.999 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:.*` matches process NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT
Aug-26 09:53:54.999 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:54.999 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.009 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS
Aug-26 09:53:55.009 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:.*` matches process NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS
Aug-26 09:53:55.010 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.010 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.023 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:STRINGTIE_STRINGTIE
Aug-26 09:53:55.023 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:STRINGTIE_STRINGTIE` matches process NFCORE_RNASEQ:RNASEQ:STRINGTIE_STRINGTIE
Aug-26 09:53:55.024 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.024 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.032 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV
Aug-26 09:53:55.032 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:BEDTOOLS_GENOMECOV` matches process NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV
Aug-26 09:53:55.033 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.033 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.043 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_FORWARD:UCSC_BEDCLIP
Aug-26 09:53:55.043 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:BEDGRAPH_TO_BIGWIG_FORWARD:UCSC_BEDCLIP` matches process NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_FORWARD:UCSC_BEDCLIP
Aug-26 09:53:55.044 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.044 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.052 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_FORWARD:UCSC_BEDGRAPHTOBIGWIG
Aug-26 09:53:55.052 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:BEDGRAPH_TO_BIGWIG_FORWARD:UCSC_BEDGRAPHTOBIGWIG` matches process NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_FORWARD:UCSC_BEDGRAPHTOBIGWIG
Aug-26 09:53:55.053 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.053 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.062 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_REVERSE:UCSC_BEDCLIP
Aug-26 09:53:55.062 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:BEDGRAPH_TO_BIGWIG_REVERSE:UCSC_BEDCLIP` matches process NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_REVERSE:UCSC_BEDCLIP
Aug-26 09:53:55.063 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.063 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.069 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_REVERSE:UCSC_BEDGRAPHTOBIGWIG
Aug-26 09:53:55.069 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:BEDGRAPH_TO_BIGWIG_REVERSE:UCSC_BEDGRAPHTOBIGWIG` matches process NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_REVERSE:UCSC_BEDGRAPHTOBIGWIG
Aug-26 09:53:55.070 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.070 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.081 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:QUALIMAP_RNASEQ
Aug-26 09:53:55.082 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:QUALIMAP_RNASEQ` matches process NFCORE_RNASEQ:RNASEQ:QUALIMAP_RNASEQ
Aug-26 09:53:55.082 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.082 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.092 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_long` matches labels `process_long` for process with name NFCORE_RNASEQ:RNASEQ:DUPRADAR
Aug-26 09:53:55.093 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:DUPRADAR` matches process NFCORE_RNASEQ:RNASEQ:DUPRADAR
Aug-26 09:53:55.094 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.094 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.103 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_BAMSTAT
Aug-26 09:53:55.109 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:RSEQC:RSEQC_BAMSTAT` matches process NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_BAMSTAT
Aug-26 09:53:55.110 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.110 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.131 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_INNERDISTANCE
Aug-26 09:53:55.132 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:RSEQC:RSEQC_INNERDISTANCE` matches process NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_INNERDISTANCE
Aug-26 09:53:55.135 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.135 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.142 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_INFEREXPERIMENT
Aug-26 09:53:55.145 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:RSEQC:RSEQC_INFEREXPERIMENT` matches process NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_INFEREXPERIMENT
Aug-26 09:53:55.145 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.145 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.156 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_JUNCTIONANNOTATION
Aug-26 09:53:55.157 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:RSEQC:RSEQC_JUNCTIONANNOTATION` matches process NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_JUNCTIONANNOTATION
Aug-26 09:53:55.158 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.158 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.167 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_JUNCTIONSATURATION
Aug-26 09:53:55.168 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:RSEQC:RSEQC_JUNCTIONSATURATION` matches process NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_JUNCTIONSATURATION
Aug-26 09:53:55.169 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.169 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.176 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_READDISTRIBUTION
Aug-26 09:53:55.176 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:RSEQC:RSEQC_READDISTRIBUTION` matches process NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_READDISTRIBUTION
Aug-26 09:53:55.177 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.177 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.187 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_READDUPLICATION
Aug-26 09:53:55.187 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*:RSEQC:RSEQC_READDUPLICATION` matches process NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_READDUPLICATION
Aug-26 09:53:55.188 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.188 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.201 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:MULTIQC_TSV_STRAND_CHECK` matches process NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_STRAND_CHECK
Aug-26 09:53:55.208 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: local
Aug-26 09:53:55.208 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local'
Aug-26 09:53:55.212 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_QUANT
Aug-26 09:53:55.213 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.213 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.218 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_TX2GENE
Aug-26 09:53:55.219 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.219 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.227 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_TXIMPORT
Aug-26 09:53:55.228 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.228 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.235 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_GENE
Aug-26 09:53:55.236 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.236 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.240 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_GENE_LENGTH_SCALED
Aug-26 09:53:55.241 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.241 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.247 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_GENE_SCALED
Aug-26 09:53:55.248 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.248 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.251 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_TRANSCRIPT
Aug-26 09:53:55.252 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.252 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.256 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_SALMON
Aug-26 09:53:55.256 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.257 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.291 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNASEQ:RNASEQ:CUSTOM_DUMPSOFTWAREVERSIONS
Aug-26 09:53:55.292 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:CUSTOM_DUMPSOFTWAREVERSIONS` matches process NFCORE_RNASEQ:RNASEQ:CUSTOM_DUMPSOFTWAREVERSIONS
Aug-26 09:53:55.292 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.292 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.353 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNASEQ:RNASEQ:MULTIQC
Aug-26 09:53:55.353 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:MULTIQC` matches process NFCORE_RNASEQ:RNASEQ:MULTIQC
Aug-26 09:53:55.354 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: pbs
Aug-26 09:53:55.354 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'pbs'
Aug-26 09:53:55.373 [main] DEBUG nextflow.Session - Workflow process names [dsl2]: DUPRADAR, NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_READDUPLICATION, RSEM_PREPAREREFERENCE, CAT_ADDITIONAL_FASTA, NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_STAR_SALMON, DESEQ2_QC_SALMON, RSEQC_READDISTRIBUTION, NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN, NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:SAMTOOLS_INDEX, NFCORE_RNASEQ:RNASEQ:CUSTOM_DUMPSOFTWAREVERSIONS, GFFREAD, RSEQC_JUNCTIONSATURATION, RSEQC_READDUPLICATION, NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_STRAND_CHECK, NFCORE_RNASEQ:RNASEQ:CAT_FASTQ, CAT_FASTQ, GUNZIP, NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES, SAMTOOLS_STATS, NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT, GUNZIP_GFF, NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_REVERSE:UCSC_BEDGRAPHTOBIGWIG, HISAT2_BUILD, NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE_SCALED, NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_BAMSTAT, TRIMGALORE, BEDTOOLS_GENOMECOV, SALMON_TX2GENE, SORTMERNA, NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_FORWARD:UCSC_BEDCLIP, NFCORE_RNASEQ:RNASEQ:DUPRADAR, UMITOOLS_PREPAREFORRSEM, SAMTOOLS_INDEX, NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV, PRESEQ_LCEXTRAP, FASTQC, NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_FORWARD:UCSC_BEDGRAPHTOBIGWIG, UCSC_BEDCLIP, NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_TXIMPORT, UCSC_BEDGRAPHTOBIGWIG, NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS, MULTIQC, GTF2BED, NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_TX2GENE, GUNZIP_GTF, SALMON_INDEX, UMITOOLS_EXTRACT, UNTAR_HISAT2_INDEX, NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK, NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_GENE, UNTAR_BBSPLIT_INDEX, SAMTOOLS_FLAGSTAT, SAMPLESHEET_CHECK, NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_GENE_SCALED, DESEQ2_QC_RSEM, GUNZIP_FASTA, NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS, NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:PICARD_MARKDUPLICATES, RSEM_PREPAREREFERENCE_GENOME, UNTAR_RSEM_INDEX, NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_JUNCTIONSATURATION, SALMON_SE_GENE_SCALED, NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_TRANSCRIPT, SALMON_QUANT, MULTIQC_CUSTOM_BIOTYPE, DESEQ2_QC_STAR_SALMON, RSEM_CALCULATEEXPRESSION, NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_TRANSCRIPT, GUNZIP_TRANSCRIPT_FASTA, MAKE_TRANSCRIPTS_FASTA, NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:FASTQC, MULTIQC_TSV_FAIL_MAPPED, SALMON_SE_GENE_LENGTH_SCALED, DESEQ2_QC, NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_INDEX, PICARD_MARKDUPLICATES, RSEM_MERGE_COUNTS, SALMON_SE_GENE, NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS, NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_TX2GENE, UNTAR, NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_FAIL_MAPPED, SALMON_SUMMARIZEDEXPERIMENT, RSEQC_INNERDISTANCE, NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_INNERDISTANCE, GTF_GENE_FILTER, RSEQC_BAMSTAT, NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_INFEREXPERIMENT, NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_SALMON, QUALIMAP_RNASEQ, RSEQC_INFEREXPERIMENT, NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT, NFCORE_RNASEQ:RNASEQ:STRINGTIE_STRINGTIE, STAR_ALIGN, MULTIQC_TSV_STRAND_CHECK, NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_TXIMPORT, NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS, RSEQC_JUNCTIONANNOTATION, NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_QUANT, SALMON_TXIMPORT, NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE, CUSTOM_GETCHROMSIZES, NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_REVERSE:UCSC_BEDCLIP, SAMTOOLS_SORT, MULTIQC_TSV_FAIL_TRIMMED, SUBREAD_FEATURECOUNTS, NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT, GUNZIP_GENE_BED, UNTAR_STAR_INDEX, UNTAR_SALMON_INDEX, HISAT2_ALIGN, NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_SORT, BBMAP_BBSPLIT, NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE_LENGTH_SCALED, RSEQC_TIN, MULTIQC_TSV_FROM_LIST, NFCORE_RNASEQ:RNASEQ:QUALIMAP_RNASEQ, UMITOOLS_DEDUP, STRINGTIE_STRINGTIE, HISAT2_EXTRACTSPLICESITES, SALMON_SE_TRANSCRIPT, NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE, NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_FAIL_TRIMMED, NFCORE_RNASEQ:RNASEQ:MULTIQC, SAMTOOLS_IDXSTATS, NFCORE_RNASEQ:RNASEQ:PRESEQ_LCEXTRAP, CUSTOM_DUMPSOFTWAREVERSIONS, STAR_GENOMEGENERATE, GUNZIP_ADDITIONAL_FASTA, NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_JUNCTIONANNOTATION, NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_GENE_LENGTH_SCALED, NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_READDISTRIBUTION
Aug-26 09:53:55.382 [main] DEBUG nextflow.Session - Ignite dataflow network (133)
Aug-26 09:53:55.382 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES
Aug-26 09:53:55.384 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK
Aug-26 09:53:55.384 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:CAT_FASTQ
Aug-26 09:53:55.384 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:FASTQC
Aug-26 09:53:55.384 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE
Aug-26 09:53:55.384 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_FAIL_TRIMMED
Aug-26 09:53:55.384 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN
Aug-26 09:53:55.388 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_SORT
Aug-26 09:53:55.389 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_INDEX
Aug-26 09:53:55.389 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS
Aug-26 09:53:55.389 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT
Aug-26 09:53:55.389 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS
Aug-26 09:53:55.389 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT
Aug-26 09:53:55.389 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_TX2GENE
Aug-26 09:53:55.389 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_TXIMPORT
Aug-26 09:53:55.389 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE
Aug-26 09:53:55.389 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE_LENGTH_SCALED
Aug-26 09:53:55.389 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE_SCALED
Aug-26 09:53:55.389 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_TRANSCRIPT
Aug-26 09:53:55.389 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_STAR_SALMON
Aug-26 09:53:55.389 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_FAIL_MAPPED
Aug-26 09:53:55.390 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:PRESEQ_LCEXTRAP
Aug-26 09:53:55.391 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:PICARD_MARKDUPLICATES
Aug-26 09:53:55.391 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:SAMTOOLS_INDEX
Aug-26 09:53:55.393 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS
Aug-26 09:53:55.393 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT
Aug-26 09:53:55.394 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS
Aug-26 09:53:55.394 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:STRINGTIE_STRINGTIE
Aug-26 09:53:55.394 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV
Aug-26 09:53:55.394 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_FORWARD:UCSC_BEDCLIP
Aug-26 09:53:55.394 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_FORWARD:UCSC_BEDGRAPHTOBIGWIG
Aug-26 09:53:55.394 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_REVERSE:UCSC_BEDCLIP
Aug-26 09:53:55.394 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_REVERSE:UCSC_BEDGRAPHTOBIGWIG
Aug-26 09:53:55.394 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:QUALIMAP_RNASEQ
Aug-26 09:53:55.394 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:DUPRADAR
Aug-26 09:53:55.394 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_BAMSTAT
Aug-26 09:53:55.395 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_INNERDISTANCE
Aug-26 09:53:55.395 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_INFEREXPERIMENT
Aug-26 09:53:55.395 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_JUNCTIONANNOTATION
Aug-26 09:53:55.395 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_JUNCTIONSATURATION
Aug-26 09:53:55.398 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_READDISTRIBUTION
Aug-26 09:53:55.399 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_READDUPLICATION
Aug-26 09:53:55.399 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_STRAND_CHECK
Aug-26 09:53:55.399 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_QUANT
Aug-26 09:53:55.399 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_TX2GENE
Aug-26 09:53:55.399 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_TXIMPORT
Aug-26 09:53:55.399 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_GENE
Aug-26 09:53:55.399 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_GENE_LENGTH_SCALED
Aug-26 09:53:55.399 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_GENE_SCALED
Aug-26 09:53:55.399 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_TRANSCRIPT
Aug-26 09:53:55.399 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_SALMON
Aug-26 09:53:55.399 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:CUSTOM_DUMPSOFTWAREVERSIONS
Aug-26 09:53:55.399 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNASEQ:RNASEQ:MULTIQC
Aug-26 09:53:55.399 [main] DEBUG nextflow.script.ScriptRunner - > Await termination 
Aug-26 09:53:55.399 [main] DEBUG nextflow.Session - Session await
Aug-26 09:53:55.527 [Actor Thread 2] DEBUG nextflow.container.SingularityCache - Singularity found local store for image=https://depot.galaxyproject.org/singularity/python:3.9--1; path=/dbs/singularity_cachedir/3.8.1/depot.galaxyproject.org-singularity-python-3.9--1.img
Aug-26 09:53:55.528 [Actor Thread 24] DEBUG nextflow.container.SingularityCache - Singularity found local store for image=https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0; path=/dbs/singularity_cachedir/3.8.1/depot.galaxyproject.org-singularity-samtools-1.15.1--h1170115_0.img
Aug-26 09:53:55.546 [Actor Thread 23] DEBUG nextflow.sort.BigSort - Sort completed -- entries: 1; slices: 1; internal sort time: 0.0 s; external sort time: 0.024 s; total time: 0.024 s
Aug-26 09:53:55.555 [Actor Thread 10] DEBUG nextflow.util.CacheHelper - Hash asset file sha-256: /test/.nextflow/assets/nf-core/rnaseq/bin/check_samplesheet.py
Aug-26 09:53:55.557 [Actor Thread 23] DEBUG nextflow.file.FileCollector - Saved collect-files list to: /scratch/37451830/329b062983f07cb1e1c82cf625cbcf86.collect-file
Aug-26 09:53:55.573 [Actor Thread 23] DEBUG nextflow.file.FileCollector - Deleting file collector temp dir: /scratch/37451830/nxf-506801252056431605
Aug-26 09:53:55.752 [Task submitter] DEBUG nextflow.executor.GridTaskHandler - [PBS] submitted process NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv) > jobId: 37451872; workDir: /test/SAMPLE/work/5d/d8adc8e9214030243ff2c7b9fddf41
Aug-26 09:53:55.752 [Task submitter] INFO  nextflow.Session - [5d/d8adc8] Submitted process > NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)
Aug-26 09:53:55.804 [Task submitter] DEBUG nextflow.executor.GridTaskHandler - [PBS] submitted process NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES (genome.fa) > jobId: 37451873; workDir: /test/SAMPLE/work/c8/d682846b11912d1242f5db6d9d8859
Aug-26 09:53:55.805 [Task submitter] INFO  nextflow.Session - [c8/d68284] Submitted process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES (genome.fa)
Aug-26 09:56:29.557 [Task monitor] DEBUG n.processor.TaskPollingMonitor - Task completed > TaskHandler[jobId: 37451872; id: 2; name: NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv); status: COMPLETED; exit: 0; error: -; workDir: /test/SAMPLE/work/5d/d8adc8e9214030243ff2c7b9fddf41 started: 1661500589545; exited: 2022-08-26T07:56:14.016725Z; ]
Aug-26 09:56:29.597 [Task monitor] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'FileTransfer' minSize=4; maxSize=4; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false
Aug-26 09:56:29.613 [Actor Thread 19] DEBUG nextflow.container.SingularityCache - Singularity found local store for image=https://depot.galaxyproject.org/singularity/trim-galore:0.6.7--hdfd78af_0; path=/dbs/singularity_cachedir/3.8.1/depot.galaxyproject.org-singularity-trim-galore-0.6.7--hdfd78af_0.img
Aug-26 09:56:29.615 [Actor Thread 4] DEBUG nextflow.container.SingularityCache - Singularity found local store for image=https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0; path=/dbs/singularity_cachedir/3.8.1/depot.galaxyproject.org-singularity-fastqc-0.11.9--0.img
Aug-26 09:56:29.879 [Task submitter] DEBUG nextflow.executor.GridTaskHandler - [PBS] submitted process NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:FASTQC (SAMPLE) > jobId: 37451900; workDir: /test/SAMPLE/work/f4/32d79b3d1d8d37c44448f42ca55297
Aug-26 09:56:29.879 [Task submitter] INFO  nextflow.Session - [f4/32d79b] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:FASTQC (SAMPLE)
Aug-26 09:56:29.930 [Task submitter] DEBUG nextflow.executor.GridTaskHandler - [PBS] submitted process NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (SAMPLE) > jobId: 37451901; workDir: /test/SAMPLE/work/35/4d7c3841f8733288bbe121c66586e7
Aug-26 09:56:29.930 [Task submitter] INFO  nextflow.Session - [35/4d7c38] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (SAMPLE)
Aug-26 09:56:59.549 [Task monitor] DEBUG n.processor.TaskPollingMonitor - Task completed > TaskHandler[jobId: 37451873; id: 1; name: NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES (genome.fa); status: COMPLETED; exit: 0; error: -; workDir: /test/SAMPLE/work/c8/d682846b11912d1242f5db6d9d8859 started: 1661500619541; exited: 2022-08-26T07:56:23.756097Z; ]
Aug-26 09:58:54.972 [Task monitor] DEBUG n.processor.TaskPollingMonitor - No more task to compute -- The following nodes are still active:
[process] NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_FAIL_TRIMMED
  status=ACTIVE
  port 0: (value) OPEN  ; channel: tsv_data
  port 1: (value) bound ; channel: header
  port 2: (value) bound ; channel: out_prefix
  port 3: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (value) bound ; channel: index
  port 2: (value) bound ; channel: gtf
  port 3: (value) bound ; channel: star_ignore_sjdbgtf
  port 4: (value) bound ; channel: seq_platform
  port 5: (value) bound ; channel: seq_center
  port 6: (value) bound ; channel: is_aws_igenome
  port 7: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_SORT
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_INDEX
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (value) bound ; channel: fasta
  port 2: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (value) bound ; channel: index
  port 2: (value) bound ; channel: gtf
  port 3: (value) bound ; channel: transcript_fasta
  port 4: (value) bound ; channel: alignment_mode
  port 5: (value) bound ; channel: lib_type
  port 6: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_TX2GENE
  status=ACTIVE
  port 0: (value) OPEN  ; channel: salmon/*
  port 1: (value) bound ; channel: gtf
  port 2: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_TXIMPORT
  status=ACTIVE
  port 0: (value) OPEN  ; channel: salmon/*
  port 1: (value) OPEN  ; channel: tx2gene
  port 2: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE
  status=ACTIVE
  port 0: (value) OPEN  ; channel: counts
  port 1: (value) OPEN  ; channel: tpm
  port 2: (value) OPEN  ; channel: tx2gene
  port 3: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE_LENGTH_SCALED
  status=ACTIVE
  port 0: (value) OPEN  ; channel: counts
  port 1: (value) OPEN  ; channel: tpm
  port 2: (value) OPEN  ; channel: tx2gene
  port 3: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_GENE_SCALED
  status=ACTIVE
  port 0: (value) OPEN  ; channel: counts
  port 1: (value) OPEN  ; channel: tpm
  port 2: (value) OPEN  ; channel: tx2gene
  port 3: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_SE_TRANSCRIPT
  status=ACTIVE
  port 0: (value) OPEN  ; channel: counts
  port 1: (value) OPEN  ; channel: tpm
  port 2: (value) OPEN  ; channel: tx2gene
  port 3: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_STAR_SALMON
  status=ACTIVE
  port 0: (value) OPEN  ; channel: counts
  port 1: (value) bound ; channel: pca_header_multiqc
  port 2: (value) bound ; channel: clustering_header_multiqc
  port 3: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_FAIL_MAPPED
  status=ACTIVE
  port 0: (value) OPEN  ; channel: tsv_data
  port 1: (value) bound ; channel: header
  port 2: (value) bound ; channel: out_prefix
  port 3: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:PRESEQ_LCEXTRAP
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:PICARD_MARKDUPLICATES
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:SAMTOOLS_INDEX
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (value) bound ; channel: fasta
  port 2: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:STRINGTIE_STRINGTIE
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (value) bound ; channel: gtf
  port 2: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_FORWARD:UCSC_BEDCLIP
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (value) bound ; channel: sizes
  port 2: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_FORWARD:UCSC_BEDGRAPHTOBIGWIG
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (value) bound ; channel: sizes
  port 2: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_REVERSE:UCSC_BEDCLIP
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (value) bound ; channel: sizes
  port 2: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:BEDGRAPH_TO_BIGWIG_REVERSE:UCSC_BEDGRAPHTOBIGWIG
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (value) bound ; channel: sizes
  port 2: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUALIMAP_RNASEQ
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (value) bound ; channel: gtf
  port 2: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:DUPRADAR
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (value) bound ; channel: gtf
  port 2: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_BAMSTAT
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_INNERDISTANCE
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (value) bound ; channel: bed
  port 2: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_INFEREXPERIMENT
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (value) bound ; channel: bed
  port 2: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_JUNCTIONANNOTATION
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (value) bound ; channel: bed
  port 2: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_JUNCTIONSATURATION
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (value) bound ; channel: bed
  port 2: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_READDISTRIBUTION
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (value) bound ; channel: bed
  port 2: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_READDUPLICATION
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_STRAND_CHECK
  status=ACTIVE
  port 0: (value) OPEN  ; channel: tsv_data
  port 1: (value) bound ; channel: header
  port 2: (value) bound ; channel: out_prefix
  port 3: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_QUANT
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: -
  port 1: (value) bound ; channel: index
  port 2: (value) bound ; channel: gtf
  port 3: (value) bound ; channel: transcript_fasta
  port 4: (value) bound ; channel: alignment_mode
  port 5: (value) bound ; channel: lib_type
  port 6: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_TX2GENE
  status=ACTIVE
  port 0: (value) OPEN  ; channel: salmon/*
  port 1: (value) bound ; channel: gtf
  port 2: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_TXIMPORT
  status=ACTIVE
  port 0: (value) OPEN  ; channel: salmon/*
  port 1: (value) OPEN  ; channel: tx2gene
  port 2: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_GENE
  status=ACTIVE
  port 0: (value) OPEN  ; channel: counts
  port 1: (value) OPEN  ; channel: tpm
  port 2: (value) OPEN  ; channel: tx2gene
  port 3: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_GENE_LENGTH_SCALED
  status=ACTIVE
  port 0: (value) OPEN  ; channel: counts
  port 1: (value) OPEN  ; channel: tpm
  port 2: (value) OPEN  ; channel: tx2gene
  port 3: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_GENE_SCALED
  status=ACTIVE
  port 0: (value) OPEN  ; channel: counts
  port 1: (value) OPEN  ; channel: tpm
  port 2: (value) OPEN  ; channel: tx2gene
  port 3: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_TRANSCRIPT
  status=ACTIVE
  port 0: (value) OPEN  ; channel: counts
  port 1: (value) OPEN  ; channel: tpm
  port 2: (value) OPEN  ; channel: tx2gene
  port 3: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_SALMON
  status=ACTIVE
  port 0: (value) OPEN  ; channel: counts
  port 1: (value) bound ; channel: pca_header_multiqc
  port 2: (value) bound ; channel: clustering_header_multiqc
  port 3: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:CUSTOM_DUMPSOFTWAREVERSIONS
  status=ACTIVE
  port 0: (queue) OPEN  ; channel: versions
  port 1: (cntrl) -     ; channel: $

[process] NFCORE_RNASEQ:RNASEQ:MULTIQC
  status=ACTIVE
  port 0: (value) bound ; channel: multiqc_config
  port 1: (value) bound ; channel: multiqc_custom_config
  port 2: (value) OPEN  ; channel: software_versions
  port 3: (queue) OPEN  ; channel: workflow_summary
  port 4: (value) OPEN  ; channel: fail_trimming_summary
  port 5: (value) OPEN  ; channel: fail_mapping_summary
  port 6: (value) OPEN  ; channel: fail_strand_check
  port 7: (value) OPEN  ; channel: fastqc/*
  port 8: (value) OPEN  ; channel: trimgalore/fastqc/*
  port 9: (value) OPEN  ; channel: trimgalore/*
  port 10: (value) bound ; channel: sortmerna/*
  port 11: (value) OPEN  ; channel: star/*
  port 12: (value) bound ; channel: hisat2/*
  port 13: (value) bound ; channel: rsem/*
  port 14: (value) OPEN  ; channel: salmon/*
  port 15: (value) OPEN  ; channel: samtools/stats/*
  port 16: (value) OPEN  ; channel: samtools/flagstat/*
  port 17: (value) OPEN  ; channel: samtools/idxstats/*
  port 18: (value) OPEN  ; channel: picard/markduplicates/*
  port 19: (value) bound ; channel: featurecounts/*
  port 20: (value) OPEN  ; channel: deseq2/aligner/*
  port 21: (value) OPEN  ; channel: deseq2/aligner/*
  port 22: (value) OPEN  ; channel: deseq2/pseudoaligner/*
  port 23: (value) OPEN  ; channel: deseq2/pseudoaligner/*
  port 24: (value) OPEN  ; channel: preseq/*
  port 25: (value) OPEN  ; channel: qualimap/*
  port 26: (value) OPEN  ; channel: dupradar/*
  port 27: (value) OPEN  ; channel: rseqc/bam_stat/*
  port 28: (value) OPEN  ; channel: rseqc/infer_experiment/*
  port 29: (value) OPEN  ; channel: rseqc/inner_distance/*
  port 30: (value) OPEN  ; channel: rseqc/junction_annotation/*
  port 31: (value) OPEN  ; channel: rseqc/junction_saturation/*
  port 32: (value) OPEN  ; channel: rseqc/read_distribution/*
  port 33: (value) OPEN  ; channel: rseqc/read_duplication/*
  port 34: (value) bound ; channel: rseqc/tin/*
  port 35: (cntrl) -     ; channel: $

Aug-26 09:58:59.565 [Task monitor] DEBUG n.processor.TaskPollingMonitor - !! executor pbs > tasks to be completed: 2 -- submitted tasks are shown below
~> TaskHandler[jobId: 37451900; id: 4; name: NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:FASTQC (SAMPLE); status: SUBMITTED; exit: -; error: -; workDir: /test/SAMPLE/work/f4/32d79b3d1d8d37c44448f42ca55297 started: -; exited: -; ]
~> TaskHandler[jobId: 37451901; id: 3; name: NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (SAMPLE); status: SUBMITTED; exit: -; error: -; workDir: /test/SAMPLE/work/35/4d7c3841f8733288bbe121c66586e7 started: -; exited: -; ]
Aug-26 09:59:29.577 [Task monitor] DEBUG n.processor.TaskPollingMonitor - Task completed > TaskHandler[jobId: 37451900; id: 4; name: NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:FASTQC (SAMPLE); status: COMPLETED; exit: 0; error: -; workDir: /test/SAMPLE/work/f4/32d79b3d1d8d37c44448f42ca55297 started: 1661500739566; exited: 2022-08-26T07:59:08.716672Z; ]
Aug-26 09:59:29.607 [Task monitor] DEBUG n.processor.TaskPollingMonitor - Task completed > TaskHandler[jobId: 37451901; id: 3; name: NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (SAMPLE); status: COMPLETED; exit: 0; error: -; workDir: /test/SAMPLE/work/35/4d7c3841f8733288bbe121c66586e7 started: 1661500739580; exited: 2022-08-26T07:59:11.26673Z; ]
Aug-26 09:59:29.747 [Task monitor] DEBUG nextflow.processor.TaskProcessor - Process NFCORE_RNASEQ:RNASEQ:FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE > Skipping output binding because one or more optional files are missing: fileoutparam<3:1>
Aug-26 09:59:29.779 [Actor Thread 40] DEBUG nextflow.sort.BigSort - Sort completed -- entries: 4; slices: 1; internal sort time: 0.009 s; external sort time: 0.001 s; total time: 0.01 s
Aug-26 09:59:29.783 [Task submitter] INFO  nextflow.Session - [4e/eee04c] Submitted process > NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_FAIL_TRIMMED
Aug-26 09:59:29.790 [Task monitor] DEBUG n.processor.TaskPollingMonitor - Task completed > TaskHandler[id: 5; name: NFCORE_RNASEQ:RNASEQ:MULTIQC_TSV_FAIL_TRIMMED; status: COMPLETED; exit: -; error: -; workDir: /test/SAMPLE/work/4e/eee04c7de2d9064333ac902203fe1a]
Aug-26 09:59:29.800 [Actor Thread 40] DEBUG nextflow.file.FileCollector - Saved collect-files list to: /scratch/37451830/046b6ca717a9b8c0a7126d980b08b6e0.collect-file
Aug-26 09:59:29.801 [Actor Thread 40] DEBUG nextflow.file.FileCollector - Deleting file collector temp dir: /scratch/37451830/nxf-6759088701034148847
Aug-26 09:59:29.858 [Actor Thread 35] DEBUG nextflow.container.SingularityCache - Singularity found local store for image=https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0; path=/dbs/singularity_cachedir/3.8.1/depot.galaxyproject.org-singularity-multiqc-1.11--pyhdfd78af_0.img
Aug-26 09:59:29.935 [Task submitter] DEBUG nextflow.executor.GridTaskHandler - [PBS] submitted process NFCORE_RNASEQ:RNASEQ:CUSTOM_DUMPSOFTWAREVERSIONS (1) > jobId: 37451994; workDir: /test/SAMPLE/work/d5/d201bf64babc5cf4a948edf4bf4037
Aug-26 09:59:29.935 [Task submitter] INFO  nextflow.Session - [d5/d201bf] Submitted process > NFCORE_RNASEQ:RNASEQ:CUSTOM_DUMPSOFTWAREVERSIONS (1)
Aug-26 10:03:09.598 [Task monitor] DEBUG n.processor.TaskPollingMonitor - Task completed > TaskHandler[jobId: 37451994; id: 6; name: NFCORE_RNASEQ:RNASEQ:CUSTOM_DUMPSOFTWAREVERSIONS (1); status: COMPLETED; exit: 0; error: -; workDir: /test/SAMPLE/work/d5/d201bf64babc5cf4a948edf4bf4037 started: 1661500989588; exited: 2022-08-26T08:02:53.567749Z; ]
Aug-26 10:03:09.652 [Actor Thread 24] DEBUG nextflow.util.CacheHelper - Hash asset file sha-256: /test/.nextflow/assets/nf-core/rnaseq/assets/multiqc_config.yml
Aug-26 10:03:09.721 [Task submitter] DEBUG nextflow.executor.GridTaskHandler - [PBS] submitted process NFCORE_RNASEQ:RNASEQ:MULTIQC (1) > jobId: 37452165; workDir: /test/SAMPLE/work/ff/9dabab6e2ee78c0aa70c443bd139a8
Aug-26 10:03:09.721 [Task submitter] INFO  nextflow.Session - [ff/9dabab] Submitted process > NFCORE_RNASEQ:RNASEQ:MULTIQC (1)
Aug-26 10:03:55.032 [Task monitor] DEBUG n.processor.TaskPollingMonitor - No more task to compute -- The following nodes are still active:
  [operator] toList

Aug-26 10:03:59.594 [Task monitor] DEBUG n.processor.TaskPollingMonitor - !! executor pbs > tasks to be completed: 1 -- submitted tasks are shown below
~> TaskHandler[jobId: 37452165; id: 7; name: NFCORE_RNASEQ:RNASEQ:MULTIQC (1); status: SUBMITTED; exit: -; error: -; workDir: /test/SAMPLE/work/ff/9dabab6e2ee78c0aa70c443bd139a8 started: -; exited: -; ]
Aug-26 10:05:44.614 [Task monitor] DEBUG n.processor.TaskPollingMonitor - Task completed > TaskHandler[jobId: 37452165; id: 7; name: NFCORE_RNASEQ:RNASEQ:MULTIQC (1); status: COMPLETED; exit: 0; error: -; workDir: /test/SAMPLE/work/ff/9dabab6e2ee78c0aa70c443bd139a8 started: 1661501114763; exited: 2022-08-26T08:05:30.657464Z; ]
Aug-26 10:05:44.648 [main] DEBUG nextflow.Session - Session await > all process finished
Aug-26 10:05:44.748 [main] DEBUG nextflow.Session - Session await > all barriers passed
Aug-26 10:05:44.754 [main] INFO  nextflow.Nextflow - -[nf-core/rnaseq] Pipeline completed successfully-
Aug-26 10:05:45.606 [main] DEBUG nextflow.trace.WorkflowStatsObserver - Workflow completed > WorkflowStats[succeededCount=7; failedCount=0; ignoredCount=0; cachedCount=0; pendingCount=0; submittedCount=0; runningCount=0; retriesCount=0; abortedCount=0; succeedDuration=11m 3s; failedDuration=0ms; cachedDuration=0ms;loadCpus=0; loadMemory=0; peakRunning=2; peakCpus=16; peakMemory=108 GB; ]
Aug-26 10:05:45.606 [main] DEBUG nextflow.trace.TraceFileObserver - Flow completing -- flushing trace file
Aug-26 10:05:45.608 [main] DEBUG nextflow.trace.ReportObserver - Flow completing -- rendering html report
Aug-26 10:05:45.638 [main] DEBUG nextflow.trace.ReportObserver - Execution report summary data:
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(samplesheet.csv)"},"memUsage":{"mean":0.22,"min":0.22,"q1":0.22,"q2":0.22,"q3":0.22,"max":0.22,"minLabel":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)","maxLabel":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)","q1Label":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)","q2Label":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)","q3Label":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)"},"timeUsage":null,"vmem":{"mean":23343104,"min":23343104,"q1":23343104,"q2":23343104,"q3":23343104,"max":23343104,"minLabel":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)","maxLabel":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)","q1Label":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)","q2Label":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)","q3Label":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)"},"reads":{"mean":1536154,"min":1536154,"q1":1536154,"q2":1536154,"q3":1536154,"max":1536154,"minLabel":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)","maxLabel":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)","q1Label":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)","q2Label":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)","q3Label":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)"},"cpu":{"mean":69.4,"min":69.4,"q1":69.4,"q2":69.4,"q3":69.4,"max":69.4,"minLabel":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)","maxLabel":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)","q1Label":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK (samplesheet.csv)","q2Label":"NFCORE_RNASEQ:RNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK 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Aug-26 10:05:46.285 [main] DEBUG nextflow.trace.TimelineObserver - Flow completing -- rendering html timeline
Aug-26 10:05:46.760 [main] DEBUG nextflow.cache.CacheDB - Closing CacheDB done
Aug-26 10:05:46.798 [main] DEBUG nextflow.script.ScriptRunner - > Execution complete -- Goodbye

System information:

Nextflow version: 22.04.5
Hardware: HPC
Executor: pbs
Container engine: Singularity
OS: CentOS Linux release 7.8.2003 (Core)
Version of nf-core/rnaseq: 3.8

Trying to re-run the pipeline with the -resume option doesn't do anything.

l0ka commented 2 years ago

Within the work dir I found a file called fail_trimmed_samples_mqc.tsv. Here's the content:

Sample  Reads after trimming
SAMPLE  0

Can this be the source of the issue?

l0ka commented 2 years ago

So, the FASTQs are the issue: after trimming there's nothing left.

drpatelh commented 2 years ago

Oooof 🤦🏽 Thanks for de-bugging @l0ka. I have created an issue for this so we can add the functionality to automatically exclude these samples in future releases https://github.com/nf-core/rnaseq/issues/862#issue-1352053147

l0ka commented 2 years ago

Thank you :)