nf-core / rnavar

gatk4 RNA variant calling pipeline
https://nf-co.re/rnavar
MIT License
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input file name collision #108

Open vivischuch opened 1 year ago

vivischuch commented 1 year ago

Description of the bug

ERROR ~ Error executing process > 'NFCORE_RNAVAR:RNAVAR:SPLITNCIGAR:SAMTOOLS_MERGE (15)'

Caused by: Process NFCORE_RNAVAR:RNAVAR:SPLITNCIGAR:SAMTOOLS_MERGE input file name collision -- There are multiple input files for each of the following file names: Non-hypertensive_Control_SRR16505214.bam

Tip: view the complete command output by changing to the process work dir and entering the command cat .command.out

-- Check '.nextflow.log' file for details Execution cancelled -- Finishing pending tasks before exit

Command used and terminal output

$ nextflow run nf-core/rnavar -profile docker -c custom.comfig --genome GRCh38 --read_length 151 --fasta GRCh38.primary_assembly.genome.fa --dbsnp All_20180418.vcf.gz  --dbsnp_tbi All_20180418.vcf.gz.tbi --known_indels Homo_sapiens_assembly38.known_indels.vcf.gz --known_indels_tbi Homo_sapiens_assembly38.known_indels.vcf.gz.tbi --input Samplesheet.csv --outdir /home/vivischuch/fastq/output_rnavar --star_index /home/vivischuch/fastq/star_files -bg -resume

Relevant files

Samplesheet.csv

System information

N E X T F L O W ~ version 23.04.4 Launching https://github.com/nf-core/rnavar [disturbed_murdock] DSL2 - revision: 222d7a15f7 [master]


                                    ,--./,-.
    ___     __   __   __   ___     /,-._.--~'

|\ | | / / \ |__) |__ } { | \| | \__, \__/ | \ |___ \-.,--, .,._,' nf-core/rnavar v1.0.0

Core Nextflow options revision : master runName : disturbed_murdock containerEngine : docker launchDir : /home/vivischuch/fastq workDir : /home/vivischuch/fastq/work projectDir : /home/vivischuch/.nextflow/assets/nf-core/rnavar userName : vivischuch profile : docker configFiles : /home/vivischuch/.nextflow/assets/nf-core/rnavar/nextflow.config, /home/vivischuch/fastq/custom.comfig

Input/output options input : Samplesheet.csv outdir : /home/vivischuch/fastq/output_rnavar

Reference genome options genome : GRCh38 fasta : GRCh38.primary_assembly.genome.fa gtf : s3://ngi-igenomes/igenomes/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf known_indels : Homo_sapiens_assembly38.known_indels.vcf.gz known_indels_tbi: Homo_sapiens_assembly38.known_indels.vcf.gz.tbi dbsnp : All_20180418.vcf.gz dbsnp_tbi : All_20180418.vcf.gz.tbi

Alignment options star_index : /home/vivischuch/fastq/star_files star_twopass : true

!! Only displaying parameters that differ from the pipeline defaults !!

maxulysse commented 1 year ago

Can you share your samplesheet?

maxulysse commented 1 year ago

Nevermind, saw it

maxulysse commented 1 year ago

Can you try with that? Samplesheet.csv I'm not sure about the space in the sample id

vivischuch commented 1 year ago

I'll try. I didn't realize there was space in my sample id. Thank you so much!

maxulysse commented 1 year ago

I'll try. I didn't realize there was space in my sample id. Thank you so much!

there should be an error for that anyway, so thanks for finding out