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nf-core / rnavar

gatk4 RNA variant calling pipeline
https://nf-co.re/rnavar
MIT License
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Strandedness #68

Open kunstner opened 2 years ago

kunstner commented 2 years ago

Description of feature

Hi,

I just wonder about the function of the Strandedness column in the sample sheet. Is this column really necessary and what is the function of it? I couldn't find any information about it in the documentation.

I ran all three possible cases (forward, reverse, unstranded) on some mouse data and got no differences between the different strandedness options.

Best, Axel

praveenraj2018 commented 2 years ago

It is not used anywhere in the process, hence it can be removed.