nf-core / scdownstream

A single cell transcriptomics pipeline for QC, integration and making the data presentable
https://nf-co.re/scdownstream
MIT License
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Implement pathway analysis using decoupler #6

Open nictru opened 2 weeks ago

nictru commented 2 weeks ago

https://decoupler-py.readthedocs.io/en/latest/index.html

gabora commented 2 weeks ago

Hello @nictru, there is a decoupler nf-core module for bulk analysis (https://nf-co.re/modules/decoupler_decoupler by @Nic-Nic and @vicpaton ) which could probably be a good starting point for this new feature. It handles multiple samples given in a matrix, so if you provide the single cell data as a matrix then it can return the enrichment scores per cells. Reach out to us if we can help, best Attila

nictru commented 2 weeks ago

Thank you very much! Will get back to you :)

grst commented 2 weeks ago

I would even argue that bulk analysis is what you want most of the time, see also this decoupler tutorial: https://decoupler-py.readthedocs.io/en/latest/notebooks/pseudobulk.html