nf-core / scdownstream

A single cell transcriptomics pipeline for QC, integration and making the data presentable
https://nf-co.re/scdownstream
MIT License
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Counts layer required for Scds and Solo is missing #91

Closed pakiessling closed 2 months ago

pakiessling commented 2 months ago

Description of the bug

Hi,

I noticed that some pipeline steps like Solo or Scds crash with the message: ValueError: counts is not a valid key in adata.layers. or equivalent.

I fixed this by creating the layer in filter.py before the doublet step. In the case of Solo everything ran through fine, however for Scds I get sort.index (No such file or directory) Not sure what is going on there.

Command used and terminal output

No response

Relevant files

scds.log

System information

No response