A method for physical delivery of DNA (likely also RNA) into cells. Gold or tungsten particles are coated with DNA and shot with a gene gun / particle gun on tissue like leaves, seedlings, callus or other types of tissue and cells. Often, in plant sciences, this method is used as a transient transformation method in protein or protein-protein interaction localization studies using binary vectors. Co-transformation of multiple vectors is possible.
Definition source: previous lab routine, used in various projects and own publications, maintenance of a respective lab device and introductions to it for multiple years.
New term name
biolistic transformation
Definition
A method for physical delivery of DNA (likely also RNA) into cells. Gold or tungsten particles are coated with DNA and shot with a gene gun / particle gun on tissue like leaves, seedlings, callus or other types of tissue and cells. Often, in plant sciences, this method is used as a transient transformation method in protein or protein-protein interaction localization studies using binary vectors. Co-transformation of multiple vectors is possible.
Definition source: previous lab routine, used in various projects and own publications, maintenance of a respective lab device and introductions to it for multiple years.
Parent term(s)
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Child term(s)
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Synonyms
particle bombardment
Xrefs
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Your ORCID
https://orcid.org/0000-0002-3879-7057