nh13 / DWGSIM

Whole Genome Simulator for Next-Generation Sequencing
GNU General Public License v2.0
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How to mark Quailty Score? #46

Closed studB closed 7 years ago

studB commented 7 years ago

Hello

I'm using dwgism to transform file,fa into file.fastq.

As you know, file.fa is a file that has only text line like AGCCAGATCCGG... Then, I put this file into dwgsim and, got file.fastq that have QC.

I wonder how dwgsim mark the QC on the file.fa. There is not reference data option in dwgsim.

What criteria do dwgism use for this process? Just, statistical inference?

I'm very very confusing.

Thank you

bdy6290@naver.com

nh13 commented 7 years ago

Take a look at the wikipedia entry for FASTQ. You'll also need to be familiar with actual NGS data, where FASTA for the reference and FASTQ for the reads are ubiquitous, so is a reasonable requirement for using dwgsim. Unfortunately, I am not paid to support dwgsim and so I will not have time to bring you up to speed. I would ask a mentor or teacher for help understanding the basics before starting on dwgsim.