I used sequencing data to align with Bowtie2 to the GTDB database, and then I used the gtdb_to_taxdump.py and acc2gtdb_tax.py scripts to generate name, nodes, and acc2tax files. However, I encountered an error when using ngsLCA to annotate the bam file: ngsLCA: ngsLCA.cpp:60: void mod_db(int, int, int2int&, int2char&, int2char&): Assertion `parent.count(out[i])==1' failed
nick-youngblut
commented
1 month ago
I used sequencing data to align with Bowtie2 to the GTDB database, and then I used the gtdb_to_taxdump.py and acc2gtdb_tax.py scripts to generate name, nodes, and acc2tax files. However, I encountered an error when using ngsLCA to annotate the bam file: ngsLCA: ngsLCA.cpp:60: void mod_db(int, int, int2int&, int2char&, int2char&): Assertion `parent.count(out[i])==1' failed