Support for comparing populations with different pool-size?

[GoogleCodeExporter]

Hello, 

It looks like there is no support in popoolation2 for comparing data with 
different pool-sizes. Do you plan on including any support for this, or do you 
simply not recommend using popoolation2 unless one has similar sample sizes for 
the two populations?

Thanks in advance!

Original issue reported on code.google.com by zjas...@gmail.com on 4 Apr 2012 at 5:54

[GoogleCodeExporter]

FST sliding offers the option for entering different pool-sizes. This however 
only has a minor influence on the results. You could test the influence of 
different pool-sizes to the parameters. 
Generally I would recommend to use high pool-sizes (>100). In this case I would 
only expect a minor influence of different pool-size to the results.

Original comment by RoKof...@gmail.com on 2 May 2012 at 11:22

[GoogleCodeExporter]

Hi, 

In addition to this, from the manual: "Most importantly the amount of DNA per 
individual in a single pool should be constant." 

Does this mean the number of reads per individual needs to be exactly the same? 
i.e. must my samples be barcoded to ensure this or will this work with Illumina 
reads from pooled DNA (the amount of DNA should be roughly similar in the 
samples we have - but does it need to be exactly constant)?

Thanks,
Jon.

Original comment by jom...@gmail.com on 30 Jul 2014 at 2:13