nloyfer / wgbs_tools

tools for working with Bisulfite Sequencing data while preserving reads intrinsic dependencies
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split_by_allele C>T SNPs at CpG sites #25

Closed rnbatra closed 1 year ago

rnbatra commented 1 year ago

Thank you for including the split_by_allele tutorial. Would this work suitably for C>T SNPs at CpG sites as well (using reads on the opposite strand for directional libraries)?

yonniejon commented 1 year ago

Hi. Yes. For C->T SNPs both at CpG sites and outside of CpG sites split_by_allele considers only reads from the opposite/bottom strand. Similarly it considers only reads from the top strand for G->A SNPs.

This information will be added to the tutorial/documentation.

rnbatra commented 1 year ago

thanks for the clarification!