Creating parameter specific (Arscin) transformation values

nolanlab / citrus

Citrus Development Code

GNU General Public License v3.0

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Creating parameter specific (Arscin) transformation values #124

Open SforR opened 6 years ago

SforR commented 6 years ago

Hi everyone,

With my (flowcytometry) data I see that some channels have an optimal Arcsin transformation that differs from the others. So I actually want to use different transformCofactors. Has some one experience with that? I have tried using a code transformCofactor = c(150,500,1000....)but that doesn't seem to do the trick.

Best,

Sam

SamGG commented 5 years ago

Hi, The main point I noticed very recently is that citrus does not manage compensation at all (@rbruggner correct me if I am wrong). Because it was designed for mass cytometry. So the FCS have to be compensated first. I will estimate how much work your request requires. Best.

SforR commented 5 years ago

Hi Sam,

I didn't know that actually, luckily I always first apply compensation on my FCS data together with some pre-gating. But thanks in adavance for looking and estimating the amount of work.

Cheers.