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nolanlab / citrus

Citrus Development Code
GNU General Public License v3.0
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Vast majority of Time column is populated with "1024" from citrus.exportClusterUI() output #86

Closed bc2zb closed 6 years ago

bc2zb commented 8 years ago

I exported clusters from my citrus analysis and almost every event's Time column is 1024. For one cluster, 16065 events have a Time value of 1024, and then 8 events have what appear to be real time values. After using the UI to export the clusters into a directory, I read in all the files to create a flowSet object. Looking at the flowFrame for each sample for each cluster reveals the odd Time value assignment.

rbruggner commented 8 years ago

There appears to be some strange default behavior in the write.FCS function of flowCore that truncates channel ranges to 1024. I've written a post to bioconductor asking if that's expected behavior or a bug.

https://support.bioconductor.org/p/75380/