I am really new in cytoscape and I am loading a .fcs on spade, select clustering parameters and finally run SPADE. I get the message "execution failed". Output dialog says "SPADE.driver(FILE_TO_PROCESS, filepattern=".fcs", out_dir=OUTPUT_DIR, cluster_cols=CLUSTERING_MARKERS, panels=PANELS, transforms=TRANSFORMS, layout=LAYOUT_FUNCTION, downsampling_samples=DOWNSAMPLED_EVENTS, downsampling_target_pctile=DOWNSAMPLING_TARGET_PCTILE, downsampling_exclude_pctile=DOWNSAMPLING_EXCLUDE_PCTILE, k=TARGET_CLUSTERS, clustering_samples=CLUSTERING_SAMPLES)
Error in SPADE.driver(FILE_TO_PROCESS, filepattern = ".fcs", out_dir = OUTPUT_DIR, :
unused argument (downsampling_samples = DOWNSAMPLED_EVENTS)
Execution halted
What I supposed have to do?
zbjornson
commented
9 years ago
Dear Zach
I am really new in cytoscape and I am loading a .fcs on spade, select clustering parameters and finally run SPADE. I get the message "execution failed". Output dialog says "SPADE.driver(FILE_TO_PROCESS, filepattern=".fcs", out_dir=OUTPUT_DIR, cluster_cols=CLUSTERING_MARKERS, panels=PANELS, transforms=TRANSFORMS, layout=LAYOUT_FUNCTION, downsampling_samples=DOWNSAMPLED_EVENTS, downsampling_target_pctile=DOWNSAMPLING_TARGET_PCTILE, downsampling_exclude_pctile=DOWNSAMPLING_EXCLUDE_PCTILE, k=TARGET_CLUSTERS, clustering_samples=CLUSTERING_SAMPLES) Error in SPADE.driver(FILE_TO_PROCESS, filepattern = ".fcs", out_dir = OUTPUT_DIR, : unused argument (downsampling_samples = DOWNSAMPLED_EVENTS) Execution halted What I supposed have to do?
Thank you in advance
John