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nolanlab / vortex

VorteX Clustering Environment - Java graphical tool for single-cell analysis, clustering and visualization. Latest release:
https://github.com/nolanlab/vortex/releases
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Unable to upload dataset #13

Open GalliES opened 6 years ago

GalliES commented 6 years ago

Hi there, I am trying to Vortex my dataset of CyTOF data. I Transformed the data in R, and exported the .fcs files (which looks fine on Cytobank or flowJo). However, when I try to import them in the latest Vortex release, the GUI identifies only a file of the size of 1, therefore preventing e any further clustering step. Any idea on why this is happening? Is it a bug or rather something from my side?

Thank you for the help!

nsamusik commented 6 years ago

Hi GalliES, could you please share the FCS files with me so that I could try them on my computer?

GalliES commented 6 years ago

Thank you again. Here are some test files: https://we.tl/Uopde8jj2e

I try to import all the parameters (blue) from line #0 til line #33. I excluded (yellow) "'patient"' and all the subsequent lines. It is interesting to notice that, by including the "'patient"'parameter, in the clustering, everything works fine. It seems to me like there is a need for some integer line.

Thank you again, if you can have a look it would be very helpful!