I was able to get the fast5 and fastq data for both replicates (modified and unmodified). However, if I understand correctly, they have to be mapped to the 4 concatenated references (Curlcake1, 2, 3 and 4), right? This means that in each sample/replicate there is a mixture of the 4 Curlcake sequences? Please correct me if I have misunderstood.
Hello,
I'm trying to test the curlcake data.
I was able to get the fast5 and fastq data for both replicates (modified and unmodified). However, if I understand correctly, they have to be mapped to the 4 concatenated references (Curlcake1, 2, 3 and 4), right? This means that in each sample/replicate there is a mixture of the 4 Curlcake sequences? Please correct me if I have misunderstood.
Thank you for your help Rania