Hi,
I got fast5 files generated with gupy from sequencing company. After I got that fast5 files, I basecalled fast5 files generated with albacore. The command is listed belloe:
read_fast5_basecaller.py --input ./multi_to_single/"$i"/ --recursive --worker_threads 10 --flowcell FLO-MIN106 --kit SQK-RNA001 --save_path $output/output_basecall_single/${i} -o fastq,fast5 -q 0 -n 0
And I got that error for every fast5 in my folder './multi_to_single/"$i"/"$j".fast5 "$j" extraction failed'.
Hi, I got fast5 files generated with gupy from sequencing company. After I got that fast5 files, I basecalled fast5 files generated with albacore. The command is listed belloe: read_fast5_basecaller.py --input ./multi_to_single/"$i"/ --recursive --worker_threads 10 --flowcell FLO-MIN106 --kit SQK-RNA001 --save_path $output/output_basecall_single/${i} -o fastq,fast5 -q 0 -n 0 And I got that error for every fast5 in my folder './multi_to_single/"$i"/"$j".fast5 "$j" extraction failed'.