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novoalab / EpiNano

Detection of RNA modifications from Oxford Nanopore direct RNA sequencing reads (Liu*, Begik* et al., Nature Comm 2019)
GNU General Public License v2.0
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Feature Extractions from Million Fast5 #27

Closed akesarwani closed 4 years ago

akesarwani commented 4 years ago

Untar results in millions of Fast5, each corresponds to one sequence. How do you deal with numerous fast5 efficiently. Do you pack them all in one.

enovoa commented 4 years ago

Hi Anil, your question is not specific to us, but rather should be aimed at SRA and/or nanopore. SRA converts the multitar into single tar with many fast5, as you have seen. You can convert them back into multifast5 if needed. Please liaise with them and/or with nanopore, as this is not an EpiNano-specific problem, but rather, about fast5 handling. thanks!