Exit: Error: Directory Hierarchy of rawdata '/home/ug1287/workspace/Hicdata_analysisi/rawdata/HJB/' is not correct. No '.fastq(.gz)' files detected

[15132522078]

Dear, When I use Hic-Pro (HiC-Pro version 3.1.0), I get the above error message, I checked the file directory structure, suffix, and keywords in the configuration file are fine. Can you give me some other suggestions to troubleshoot bugs? Attached at the end is my code and config file. Best wishes！

ug1287@gs142:~/workspace/Hicdata_analysisi$ HiC-Pro -i rawdata/HJB/ -o 03.result/ -c software/HiC-Pro_3.1.0/config-hicpro.txt
Exit: Error: Directory Hierarchy of rawdata '/home/ug1287/workspace/Hicdata_analysisi/rawdata/HJB/' is not correct. No '.fastq(.gz)' files detected
ug1287@gs142:~/workspace/Hicdata_analysisi$ ls /home/ug1287/workspace/Hicdata_analysisi/rawdata/HJB/
SR01_R1.fastq  SR01_R2.fastq
ug1287@gs142:~/workspace/Hicdata_analysisi$ cat software/HiC-Pro_3.1.0/config-hicpro.txt
# Please change the variable settings below if necessary

#########################################################################
## Paths and Settings  - Do not edit !
#########################################################################

TMP_DIR = tmp
LOGS_DIR = logs
BOWTIE2_OUTPUT_DIR = bowtie_results
MAPC_OUTPUT = hic_results
RAW_DIR = rawdata

#######################################################################
## SYSTEM AND SCHEDULER - Start Editing Here !!
#######################################################################
N_CPU = 70
SORT_RAM = 10000M
LOGFILE = hicpro.log

JOB_NAME = 3DGenome
JOB_MEM = 240gb
JOB_WALLTIME =
JOB_QUEUE =
JOB_MAIL =

#########################################################################
## Data
#########################################################################

PAIR1_EXT = _R1
PAIR2_EXT = _R2

[DiegoSafian]

I had the same problem. check this: https://groups.google.com/g/hic-pro/c/PrX00iA0N1U The data must be ordered like: data ---sample1 ------f1_R1.fastq ------f1_R2.fastq