nservant / HiC-Pro

HiC-Pro: An optimized and flexible pipeline for Hi-C data processing
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make: *** No targets. Stop. #603

Open AlcaArctica opened 7 months ago

AlcaArctica commented 7 months ago

I am not sure, what is going on here. This is the first time I am trying to run hic-pro, so it might be a set-up issue? I am running:

/lustre/projects/dazzler/uelze/sw/HiC-Pro/bin/HiC-Pro -i /lustre/projects/dazzlerAssembly/asm_vpTaxBacc_BK34-6/hic/hic_pro/hic_data/ -o /lustre/projects/dazzlerAssembly/asm_vpTaxBacc_BK34-6/hic/hic_pro/test -c /lustre/projects/dazzler/uelze/pipelines/hifi_snake/config_hicpro.txt -s mapping

and the only ouput I get is: make: *** No targets. Stop.

I am pretty sure I have the input folder set up right:

hic_data/
├── input_linked
│   ├── sample_ln_R1.fastq.gz -> /lustre/projects/dazzlerAssembly/asm_vpTaxBacc_BK34-6/hic/linked_reads/R1.fastq.gz
│   └── sample_ln_R2.fastq.gz -> /lustre/projects/dazzlerAssembly/asm_vpTaxBacc_BK34-6/hic/linked_reads/R2.fastq.gz
└── input_real
    ├── sample_R1.fastq.gz
    └── sample_R2.fastq.gz

2 directories, 4 files

so that should be fine. Then there is the config file:

# Please change the variable settings below if necessary

#########################################################################
## Paths and Settings  - Do not edit !
#########################################################################

TMP_DIR = tmp
LOGS_DIR = logs
BOWTIE2_OUTPUT_DIR = bowtie_results
MAPC_OUTPUT = hic_results
RAW_DIR = rawdata

#######################################################################
## SYSTEM AND SCHEDULER - Start Editing Here !!
#######################################################################
N_CPU = 40
SORT_RAM = 5000M
LOGFILE = hicpro.log

JOB_NAME = 
JOB_MEM = 
JOB_WALLTIME = 
JOB_QUEUE = 
JOB_MAIL = 

#########################################################################
## Data
#########################################################################

PAIR1_EXT = _R1
PAIR2_EXT = _R2

#######################################################################
## Alignment options
#######################################################################

MIN_MAPQ = 10

BOWTIE2_IDX_PATH = /lustre/projects/dazzlerAssembly/asm_vpTaxBacc_BK34-6/hic/hic_pro/annotation_files
BOWTIE2_GLOBAL_OPTIONS = --very-sensitive -L 30 --score-min L,-0.6,-0.2 --end-to-end --reorder
BOWTIE2_LOCAL_OPTIONS =  --very-sensitive -L 20 --score-min L,-0.6,-0.2 --end-to-end --reorder

#######################################################################
## Annotation files
#######################################################################

REFERENCE_GENOME = vpTaxBacc_BK34-6
GENOME_SIZE = /lustre/projects/dazzlerAssembly/asm_vpTaxBacc_BK34-6/hic/hic_pro/annotation_files/vptaxbacc_bk34-6.hic.hap1.p_ctg.sizes.genome

#######################################################################
## Allele specific analysis
#######################################################################

ALLELE_SPECIFIC_SNP = 

#######################################################################
## Capture Hi-C analysis
#######################################################################

CAPTURE_TARGET =
REPORT_CAPTURE_REPORTER = 1

#######################################################################
## Digestion Hi-C
#######################################################################

GENOME_FRAGMENT = /lustre/projects/dazzlerAssembly/asm_vpTaxBacc_BK34-6/hic/hic_pro/annotation_files/vptaxbacc_bk34-6.hic.hap1.p_ctg.restr.bed
LIGATION_SITE = AAGCTAGCTT
MIN_FRAG_SIZE = 
MAX_FRAG_SIZE =
MIN_INSERT_SIZE =
MAX_INSERT_SIZE =

#######################################################################
## Hi-C processing
#######################################################################

MIN_CIS_DIST =
GET_ALL_INTERACTION_CLASSES = 1
GET_PROCESS_SAM = 0
RM_SINGLETON = 1
RM_MULTI = 1
RM_DUP = 1

#######################################################################
## Contact Maps
#######################################################################

BIN_SIZE = 20000 40000 150000 500000 1000000
MATRIX_FORMAT = upper

#######################################################################
## Normalization
#######################################################################
MAX_ITER = 100
FILTER_LOW_COUNT_PERC = 0.02
FILTER_HIGH_COUNT_PERC = 0
EPS = 0.1

Which I think I filled in correctly? So what is causing the error?

Woogle90 commented 3 months ago

Hi, have you solved this? I have the similiar problem

AlcaArctica commented 3 months ago

nope, unfortunately not.

Woogle90 commented 3 months ago

I solved this but I am not sure the problem I encountered was as same as yours.

I am afraid that this is still a problem of PATH. Perhaps try to move all your files (including config-system.txt) to the directory where you run Hi-C Pro. And you might need to have a look at config-system.txt too.