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The Ontology for Biomedical Investigations
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Antibody tagged with a reporter molecule #115

Closed obi-bot closed 7 years ago

obi-bot commented 16 years ago

An antibody tagged with a reporter molecule (an enzyme or fluorophore) to enable specific protein identification in both in vitro (eg. Western Blotting) and in vivo assays (eg. Immunohistochemistry). I am not certain if the parent term should be 'polypeptide' or 'antibody'.

Reported by: ksnyder77

Original Ticket: "obi/obi-terms/115":https://sourceforge.net/p/obi/obi-terms/115

obi-bot commented 16 years ago

To answer the question, I would suggest using a polypeptide (CHEBI_15841, imported in OBI) which bears the role of antibody role (OBI_0000053)

To represent a polypeptide bearing role antibody and tagged with an enzyme which plays a reporter role:

a labeling process (OBI_0600038) (maybe specific labeling, OBI_0600067?) has_specified_input polypeptide and utilizes_reagent enzyme which bears role reporter reagent role (OBI_0000140)

this labeling process has_specified_output material_entity (polypeptide?) which has_part polypeptide which has quality labeled (OBI_0302873) (note, we still need to discuss this types of qualities acquired via a process) and has_part enzyme (in OBI file, awaiting ID) which bears role reporter reagent role.

I am assigning this to Bjoern who is our process expert.

Thanks, Melanie

Original comment by: mcourtot

obi-bot commented 16 years ago

Original comment by: mcourtot

obi-bot commented 15 years ago

Original comment by: bpeters42

obi-bot commented 15 years ago

This should be done together with Flow cytometry stuff in general. There is a corresponding ticket for MC

Original comment by: bpeters42

obi-bot commented 13 years ago

We have removed antibody role, as antibodies are well defined protein complexes. We have imported them from GO now and this should be used with the somewhat unfortunate name: "immunoglobulin complex, circulating' (alternative term is'antibody'). This should be the parent. The tagging with a reporter constitutes a labeling process, which this should be the output of. Closing the ticket, as I believe that addresses it.

Original comment by: bpeters42

obi-bot commented 13 years ago

Original comment by: bpeters42