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The Ontology for Biomedical Investigations
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NTR for CRISPR screen assays #1784

Open jenjou opened 1 month ago

jenjou commented 1 month ago

This issue covers 3 different types of CRISPR screens.

Related publications:

Preferred Term: CRISPR perturbation screen Definition: An assay in which cells expressing a Cas system are infected with a library of guide RNAs with the intent of causing a perturbation at the target of the guide RNAs. Parent Term: Somewhat related to OBI:0003133 cas mediated mutagenesis, but the cells are affected by a perturbation rather than a mutation.

Preferred Term: CRISPR perturbation screen followed by single-cell RNA sequencing Definition: An assay in which cells expressing a Cas system are infected with a library of guide RNAs with the intent of causing a perturbation at the target of the guide RNAs, and then are sequenced using single-cell RNA sequencing. Parent Term: Possibly OBI:0003133 cas mediated mutagenesis (see previous comment), OBI:0002631 single-cell RNA sequencing assay.

Preferred Term: CRISPR perturbation screen followed by flow cytometry and FISH Definition: An assay in which cells expressing a Cas system are infected with a library of guide RNAs with the intent of causing a perturbation at the target of the guide RNAs, and then are sorted using fluorescence imaging. Parent Term: Possibly OBI:0003133 cas mediated mutagenesis (see previous comment), OBI:0002960 fluorescence imaging-based reporter gene assay

DanBerrios commented 2 weeks ago

@jenjou Are you available to join one of our development meetings?

jenjou commented 2 weeks ago

Could you let me know when these meetings usually occur? I will try to join if there aren't prior conflicts for me.

jenjou commented 1 week ago

@DanBerrios Hello, I would be happy to join assuming I don't have a prior conflict. Would you be able to let me know when the next development meeting is?

jamesaoverton commented 1 week ago

OBI has weekly developer calls on Mondays. The time and connection details are at the bottom of our homepage: http://obi-ontology.org/. Looking forward to meeting your!