Closed obi-bot closed 7 years ago
obi-bot
commented
14 years ago This sounds like a cross product between an objective (detection of material located_in intracellular) and a method of assay (flow cytometry) Is that how this would be created? Should we create a flow cytometry assay has_objective intracellular material identification or has_objective cell population detection/quantification?
Original comment by: mcourtot
obi-bot
commented
14 years ago We don't even have flow cytometry assay at this point. The differentia to standard flow is in how the cells are labeled (after permeabilizing their membranes in this case), so there is a methodology difference to standard flow as well. Maybe 'surface staining' vs. 'intracellular staining' flow cytometry? I think in both cases, the objective as you said is 'cell population detection/quantification.
Original comment by: bpeters42
obi-bot
commented
14 years ago We added this to the file, and also added a flow cytometry assay parent. There definitely need to be other children. Check it out there.
Original comment by: bpeters42
obi-bot
commented
14 years ago Original comment by: bpeters42
We need this as a parent for intracellular cytokine staining for non-cytokines (e.g. perforin). Was previously requested in editor note.
Definition: An assay in which the presence of a material inside a cell is measured by permeabilizing the cell membrane to allow entry of specific antibodies, and counting the stained cells using a flow cytometer.
Reported by: bpeters42
Original Ticket: obi/obi-terms/520