Closed obi-bot closed 7 years ago
Original comment by: bpeters42
Original comment by: bpeters42
Hi,
Noticed that this term may not yet have been added? Please let me know if I missed something.
Cheers, Rob
Original comment by: rnash
Reworded definition: An assay that detects differences in protein abundance using samples that have been metabolically labeled in vivo with a stable non-radioactive heavy isotope containing amino acid for a short period of time. After diluting the pulsed cells into growth media without label, high resolution mass spectrometry-based proteomics is used to analyze the time-dependent decay and determine protein stability.
Original comment by: bpeters42
Original comment by: jamesaoverton
This term was added as OBI_0002132 in commit 4091, and will be included in the next release.
Original comment by: jamesaoverton
Thanks again! Rob
Original comment by: rnash
Hi,
I would like to request a new assay term for use at the Saccharomyces Genome Database (SGD).
editor preferred term: pulse stable isotope labeling by amino acids in cell culture alternative terms: pulse SILAC; pulsed SILAC; dynamic SILAC textual definition: A mass spectrometry-based technique that detects differences in protein abundance using samples that have been metabolically labeled in vivo with a stable non-radioactive heavy isotope containing amino acid for a short period of time. After diluting the pulsed cells into growth media without label, high resolution mass spectrometry-based proteomics is used to analyze the time-dependent decay and determine protein stability definition source for the textual definition: Schwanhausser et al., 2009 (PMID: 19053139); Frolich et al. 2009 (PMID: 18954100); term editor: Rob Nash
Thanks, Rob
Reported by: rnash
Original Ticket: obi/obi-terms/823