NTRs: nuclear ligation assay children

[beckyjackson]

circularized chromosome conformation capture assay A nuclear ligation assay that captures interactions between one locus and all other genomic loci. 3C protocol is followed until a second ligation step is added to create self-circularized DNA fragments, followed by inverse PCR to amplify an unknown sequence ligated to the known sequence. [url:https://en.wikipedia.org/wiki/Chromosome_conformation_capture] subClassOf: 'nuclear ligation assay' (also 4C assay, chromosome conformation capture on chip assay)

chromosome conformation capture sequencing assay A circularized chromosome conformation capture assay quantitates the products with multiplexed high-throughput sequencing. [PMID:23411633] subClassOf: 'circularized chromosome conformation capture assay' (or 'sequencing assay' - there should be some link) (also 3C-seq assay)

inverse polymerase chain reaction A polymerase chain reaction which amplifies DNA with only one known sequence from which primers may be designed. A series of restriction digestions and ligations result in a looped DNA fragment which can be primed for PCR. [url:https://en.wikipedia.org/wiki/Chromosome_conformation_capture] subClassOf: polymerase chain reaction

Also, it seems like 'carbon-copy chromosome conformation capture assay' (OBI:0001919) could also be a child of 'nuclear ligation assay' instead of 'sequencing assay'.

[beckyjackson]

'circularized chromosome conformation capture assay' has been added as OBI:0002458

I will create a PR with the other two.

[beckyjackson]

Terms added in c200a8d