Open GullyAPCBurns opened 6 years ago
I think 'addition of molecular label' is a perfect fit. I would change the definition to:
DEFINITION: Epitope tagging is an addition of molecular label process in which DNA encoding for an antibody epitope is added to DNA encoding for a protein so that the expressed protein product has an epitope linked to it that can be detected by antibodies specific for the epitope.
On Thu, Jul 5, 2018 at 1:04 PM, Gully Burns notifications@github.com wrote:
PROPOSED ACTION IN OBI: Add a new term with label 'Epitope Tagging' as a child of 'Material Processing'
- need to figure out if this process is a child of (A) 'addition of molecular label', (B) 'adding a material entity into a target' or some other subtype of material processing.
DEFINITION: Epitope tagging is a recombinant DNA method by which a protein encoded by a cloned gene is made immunoreactive to a known antibody.
SOURCES:
First Description: Munro, S. & Pelham, H. R. Use of peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila hsp 70. EMBO J 3, 3087–3093 (1984). https://www.ncbi.nlm.nih.gov/pubmed/6526011
Full Review: Jarvik, J. W. & Telmer, C. A. Epitope tagging. Annu Rev Genet 32, 601–618 (1998). doi:10.1146/annurev.genet.32.1.601, https://www.ncbi.nlm.nih.gov/ pubmed/9928493
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Just to make the English a little easier to read:
Epitope tagging is an "addition of molecular label" process in which the DNA encoding for an antibody epitope (X) is added to the DNA encoding for a protein (Y) in a cell. If the protein X is then expressed, then it may be detected by antibodies specific for the epitope Y.
I would remove 'in a cell', which is often not true. Otherwise great!
On Thu, Jul 5, 2018, 4:17 PM Gully Burns notifications@github.com wrote:
Just to make the English a little easier to read:
Epitope tagging is an "addition of molecular label" process in which the DNA encoding for an antibody epitope (X) is added to the DNA encoding for a protein (Y) in a cell. If the protein X is then expressed, then it may be detected by antibodies specific for the epitope Y.
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Great! Here's the latest definition:
Epitope tagging is an "addition of molecular label" process in which the DNA encoding for an antibody epitope (X) is added to the DNA encoding for a protein (Y). If the protein X is then expressed, then it may be detected by antibodies specific for the epitope Y.
Flipped X and Y in the second sentence :) I also don't think it is necessary:
On Fri, Jul 6, 2018 at 9:56 AM, Gully Burns notifications@github.com wrote:
Great! Here's the latest definition:
Epitope tagging is an "addition of molecular label" process in which the DNA encoding for an antibody epitope (X) is added to the DNA encoding for a protein (Y). If the protein X is then expressed, then it may be detected by antibodies specific for the epitope Y.
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-- Bjoern Peters Professor La Jolla Institute for Allergy and Immunology 9420 Athena Circle La Jolla, CA 92037, USA Tel: 858/752-6914 Fax: 858/752-6987 http://www.liai.org/pages/faculty-peters
From Becky:
An "addition of molecular label" process in which the DNA encoding for an antibody epitope (X) is added to the DNA encoding for a protein (Y). If the protein Y is then expressed, then it may be detected by antibodies specific for the epitope X.
Great feedback on the call for this process. Thanks Guys!
Hi @GullyAPCBurns - do we still want to add this term as a child of "addition of molecular label"? It looks like it has a home and a definition.
PROPOSED ACTION IN OBI: Add a new term with label 'Epitope Tagging' as a child of 'Material Processing'
DEFINITION: Epitope tagging is a recombinant DNA method by which a protein encoded by a cloned gene is made immunoreactive to a known antibody.
SOURCES:
First Description: Munro, S. & Pelham, H. R. Use of peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila hsp 70. EMBO J 3, 3087–3093 (1984). https://www.ncbi.nlm.nih.gov/pubmed/6526011
Full Review: Jarvik, J. W. & Telmer, C. A. Epitope tagging. Annu Rev Genet 32, 601–618 (1998). doi:10.1146/annurev.genet.32.1.601, https://www.ncbi.nlm.nih.gov/pubmed/9928493