weshorton
commented
7 years ago To Do
- Turn into label
- Milestones
- amplicon bias (normalization)
- sample normalization
- batch normalization
- Identify issues related to this label.
- Re-visit when project is correctly redefined and determine dependencies between above milestones.
Summary
We have multiple batches that are run at different times and with different total amounts of DNA placed on the sequencer. We need to be able to normalize the differences in read depth across batches in order to be able to compare samples.
Significance
Across batch normalization is extremely important for our project so that multiple experiments can be compared in order to create a more robust picture of TCR repertoire diversity.
To Do
Need to complete our amplification bias normalization first before we are able to tackle this problem.
Relevant Information
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