Open alexandrebastien opened 6 months ago
This issue has been mentioned on Image.sc Forum. There might be relevant details there:
https://forum.image.sc/t/wrong-units-from-tem-diffraction-image-of-gatan-camera-dm4/92349/7
Thanks, @alexandrebastien. Transferred to the https://github.com/ome/ome-model repo, as this is something that would need to be updated in the OME schema (specifically, the UnitsLength
enum). Once the schema is updated, Bio-Formats would be able to make use of the new units more or less automatically.
Transmission electron microscope can produce diffraction image often called SAED. These would be in “1/nm” units since they are from the conjugated plane. When we open such a file (*.dm4) in Fiji with Bio-Format, the following warning is displayed:
[WARN] Not adjusting for unknown units: 1/nm
If we go in “scale bar”, the unit was simply replaced with microns, which is misleading and should not be used. I suggest that the 1/nm unit could simply be included as a valid unit in Bio-Format. It's often presented with parenthesis to avoid confusion "(1/nm)".Here's an exemple of how an analysis of these images could be done. Briefly, length are kept in reciprocal (1/nm) units, then the user would invert them to obtain the d-spacing which is the wanted value is those analysis.
In my case, the camera used to produce the images is a Gatan OneView, filetype is dm4, Fiji/ImageJ 2.14.0/1.54f; Java 1.8.0_322 [64-bit]; Windows 10