omnideconv / SimBu

Simulate pseudo-bulk RNAseq samples from scRNAseq expression data
http://omnideconv.org/SimBu/
GNU General Public License v3.0
12 stars 1 forks source link

Error: BiocParallel errors 1 remote errors, element index: 1 #46

Open MengjurJ opened 1 year ago

MengjurJ commented 1 year ago

Hi,when I was ready to simulate the pseudo bulk samples using 'custom' scenario, it reported an error.

Error: BiocParallel errors 1 remote errors, element index: 1 1 unevaluated and other errors first remote error: invalid 'type' (character) of argument

mycode:

simulation <- SimBu::simulate_bulk(data = ds, scenario = "custom", custom_scenario_data = cell_pt0, scaling_factor = "NONE", ncells=3000, nsamples = 2, BPPARAM = BiocParallel::MulticoreParam(workers = 4), run_parallel = F) ############ However, I can run the next code successfully when use the same data

mycode2:

simulation <- SimBu::simulate_bulk(data = ds, scenario = "random", custom_scenario_data = cell_pt0,
scaling_factor = "NONE", ncells=2000, nsamples = 1, BPPARAM = BiocParallel::MulticoreParam(workers = 4),
run_parallel = TRUE)

Now,how to deal with the problem?

alex-d13 commented 1 year ago

Hi,

Can you show me, how your cell_pt0 object looks like? Maybe there is something wrong with it :)

MengjurJ commented 1 year ago

cell_pt0 is a data.frame, as follows:

#              B.cell  CD8.T    DCs Macrophage Monocyte     NK Plasma activate.CD4.T naive.CD4.T
#sample1 0.1242 0.1366 0.0426     0.1068   0.2130 0.0976 0.0132         0.1636      0.1024