MiSET RFC Standards - A tool for objective assessment of experimental techniques and methods

[FabLucas]

Project Lead: Fabienne Lucas, MDPhD, Resident in Clinical Pathology Brigham and Women's Hospital Boston, MA, Pathology Fellow Harvard Medical School Boston MA @FabLucas

Mentor: @malvikasharan

Welcome to OLS-3! This issue will be used to track your project and progress during the program. Please use this checklist over the next few weeks as you start Open Life Science program :tada:.

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Week 1 (week starting 8 February 2021): Meet your mentor!

• [x] Create an account on GitHub
• [x] Check if you have access to the HackMD notes set up for your meetings with your mentor
• [x] Prepare to meet your mentor(s) by completing a short homework provided in the HackMD notes
• [x ] Complete your own copy of the open leadership self-assessment and share it to your mentor If you're a group, each teammate should complete this assessment individually. This is here to help you set your own personal goals during the program. No need to share your results, but be ready to share your thoughts with your mentor.
• [x ] Make sure you know when and how you'll be meeting with your mentor.

Before Week 2 (week starting 15 February 2021): Cohort Call (Welcome to Open Life Science!)

• [x ] Create an issue on the OLS-3 GitHub repository for your OLS work and share the link to your mentor.

• [x ] Draft a brief vision statement using your goals

  This lesson from the Open Leadership Training Series (OLTS) might be helpful

• [x ] Leave a comment on this issue with your draft vision statement & be ready to share this on the call

• [x ] Check the Syllabus for notes and connection info for all the cohort calls.

Before Week 3 (week starting 22 February 2021): Meet your mentor!

• [x] Look up two other projects and comment on their issues with feedback on their vision statement
• [ ] Complete this compare and contrast assignment about current and desired community interactions and value exchanges
• [x] Complete your Open Canvas (instructions, canvas)
• [ ] Share a link to your Open Canvas in your GitHub issue
• [x] Start your Roadmap
• [ ] Comment on your issue with your draft Roadmap
• [x] Suggest a cohort name at the bottom of the shared notes and vote on your favorite with a +1

Before Week 4 (week starting 1 March 2021): Cohort Call (Tooling and roadmapping for Open projects)

• [ ] Look up two other projects and comment on their issues with feedback on their open canvas.

Week 5 and later

• [ ] Create a GitHub repository for your project
• [ ] Add the link to your repository in your issue
• [ ] Use your canvas to start writing a README.md file, or landing page, for your project
• [ ] Link to your README in a comment on this issue
• [ ] Add an open license to your repository as a file called LICENSE.md
• [ ] Add a Code of Conduct to your repository as a file called CODE_OF_CONDUCT.md
• [ ] Invite new contributors to into your work!

This issue is here to help you keep track of work as you start Open Life Science program. Please refer to the OLS-3 Syllabus for more detailed weekly notes and assignments past week 4.

[FabLucas]

MiSET RFC (Minimum Set of Research Flow Cytometry) vision statement: The irreproducibility of published scientific findings is a global economic and ethical challenge. Metrics to evaluate flow cytometry data quality were published as MiFlowCyt in 2008, and the FlowRepository was created for data deposition. Neither of these resources have found widespread adoption. Alarmingly, incentives to demonstrate cytometry best practices are absent during the peer-review process, and most journals do not require data deposition or authentication of controls, reagents, and instrumentation.

MiSET MISSION: The MiSET initiative aims to translate the minimum set of experimental quality standards into a quality assessment tool that evaluates the technical aspects of cytometry publications, and to fully integrate these flow cytometry standards into grant submission and publication requirements across scientific fields.

MiSET RFC Standards - designing a data, user and expert-based tool for objective assessment of experimental techniques and methods - reducing human error in scientific peer-review and publishing

[FabLucas]

Lucas F CytometryA 2019_MiSET RFC Standards.pdf

Our publication in 2019 outlining the background, need and roadmap of MiSET

[FabLucas]

Lucas et al_CYTO2020_Poster _MiSET Tool.pdf

Our latest poster from the 2020 CYTO conference providing a preliminary analysis of the community survey

[FabLucas]

This is the link to our flyer

https://drive.google.com/file/d/11gzFmj91gp6vkPkYYz5KMlBYHrBusEpG/view?usp=sharing

[spitschan]

Commenting on here to provide feedback as per the OLS-3 instruction – feel free to delete post since I don't want to clutter up your space. While I've never worked with flow cytometry methods or data, this is a really cool project and I can imagine many ways that the process you describe could be quite useful for our team's project as well (Chronobiology Data Standards Group, here's the vision statement).

[FabLucas]

Commenting on here to provide feedback as per the OLS-3 instruction – feel free to delete post since I don't want to clutter up your space. While I've never worked with flow cytometry methods or data, this is a really cool project and I can imagine many ways that the process you describe could be quite useful for our team's project as well (Chronobiology Data Standards Group, here's the vision statement).

Hello @spitschan Christine Blume told me a lot about your project when we were in the same breakout room last week - I was thinking the same thing, and wanted to reach out to you guys. but you beat me to it! Do you want to set up a zoom for some brainstorming? We are now moving towards survey publication, but are struggling with the metrics and the tool development. Our group has zero expertise in those things unfortunately...

[spitschan]

Great! Looping @ChristineBlume into this thread. Would be good to find a time to Zoom.