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Connecting Luer Ibidi µSlide to Tubes #5

Closed beniroquai closed 2 months ago

beniroquai commented 3 months ago

Hey @ranranking do you know any good/standardized tubing system for the microslide?

ranranking commented 3 months ago

i think the tube is always standard FEP 1/8 tube i think, maybe we can try with the option from ibidi?

https://ibidi.com/flow-accessories/75-tube-adapter-set.html

https://ibidi.com/flow-accessories/68-elbow-luer-connector.html

beniroquai commented 3 months ago

Thanks. Now we need to think about adapting it to the valves and the pump before we connect it to the ferry box. What's the connector here?

beniroquai commented 3 months ago

@ranranking are there alternative solutions? How did You connect the tubing back then with the tweezers? Which glue would hold? Hot glue doesn't ;)

christiankuttke commented 3 months ago

The connector of the Ibidi slide is Luer (no lock) female. Luer Lock male may fit in and over its ports: https://www.mcmaster.com/51525K273/ With the Luer adapter kit from ibidi, there are elbows shown that have a big square opposite of where they plug in to the slide. A 3D-printed slide holder assembly could put pressure on the blocks with a printed spring structure that is put under tension when the slide is screwed into the 3D-printed assembly.

The plumbing I am speccing with Luer Lock connectors everywhere. Plumbing parts like check valves, stop valves and Tees are very commonly available as discussed here: https://github.com/openUC2/openUC2_SOOP/issues/9#issuecomment-2231062810

Tubing: A good size to me seems to be 1/8 inch (3.1 mm). Fitting (gender is an example, material and type is target): https://www.mcmaster.com/51525K273/ The fitting uses 1 barb of 0.19" OD (4.826 mm) at the widest diameter. 1/8" ID tube would have 0.125" ID (0.125 mm). If the fitting is designed to stretch so much over the barb, then the metric tube will also work. I think the fittings don't need to be fastened beyond being pushed into the tube.

christiankuttke commented 3 months ago

Here is the Ibidi µ-slide Luer product information datasheet: https://ibidi.com/img/cms/products/labware/channel_slides/S_801XX_Slide_ILuer/IN_801XX_I_Luer.pdf

christiankuttke commented 3 months ago

i think the tube is always standard FEP 1/8 tube i think, maybe we can try with the option from ibidi?

https://ibidi.com/flow-accessories/75-tube-adapter-set.html

https://ibidi.com/flow-accessories/68-elbow-luer-connector.html

Ibidi adapter sets or at least the elbow is for 1.6 mm tubing! (1/16" ID) It should not be used if we use 1/8" or 3 mm tubing for all of our plumbing. We can work something out and get our own Luer male fittings to connect to the ibidi slide.

beniroquai commented 3 months ago

Ok. Thanks @christiankuttke for all the details. I guess I get the current situation roughly. I like the Elbow connectors with the right tubing. Would you mind to make or enhance the drawing you created in the other post with part numbers and/or names? In best case all components com from one manufacturer so that we keep the BOM simple.

From what I understand:

We start with gardina tubing from the boat-side. Then we have a bypass that will go to our system. Our system has yet another bypass where we have a "shunt" (0resistance kinda thing) and the microscope. The microscope has the chip that needs luer (1ml syringes actually work too..didn't expect it was luer..), then from luer we need a tube to the pump that requires 2x4/3x5mm tubes according to the "manufacturer" https://www.aliexpress.us/item/3256804054017181.html From there we would need another 2x4/3x5mm tube to the T that closes the bypass. We would need the valves too. Would you mind creating such diagram e.g. in Powerpoint or something similar and post the link here? That would be brilliant! Thanks!

christiankuttke commented 3 months ago

I will create a bill of material and create diagrams later this week. I will also include tubing and the adapters and fittings to make it work with gardena.

Is our connection to the boat a single gardena connector or two (inlet/outlet)?

If there is a continuous line where we have a single Tee off of, and if there are other measurements done with the water downstream from us, our flushing will introduce "old" water since it is stored in our box and then later used to flush our flowcell.

beniroquai commented 3 months ago

That's a very good question. We should get back to the Helmholtz people! Do you add all these information alongside the other design freeze decisions into the Readme.md? That would be great! Thanks! :)

beniroquai commented 2 months ago

I have ordered all the parts. Let's now go over and put the parts together once they arrive @christiankuttke.