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pFindStudio / pGlyco3

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Difficulty locating a single HexNAc (1) in the protein modification list #24

Closed AnnMaria-J closed 1 year ago

AnnMaria-J commented 1 year ago

Hello!

My lab is trying to use pGlyco to locate N-glycosylation as a result of the enzyme EndoH. This leaves a single HexNAc on N-asparagine. Unfortunately, we can't find the setting for a single HexNAc under the protein modification list or any other list. Are we looking in the wrong spot or does the program automatically modify for single HexNAcs? Any help would be greatly appreciated! I've attached images of all the protein modifications for Hex, in case it is there but I simply haven't been able to identify it.

Thanks!

hexNac1 hexNac2

AnnMaria-J commented 1 year ago

We found it labeled as HexNAc [N] underneath all of the Hex options. It wasn't necessarily with all of the other HexNAc options, which is was threw us off.