Open WeilerP opened 2 years ago
kallisto | bustools will return non-integer counts >=0 if ambiguous 3' reads are disambiguated with the EM algorithm. Practically speaking this happens if bustools count --em
is chosen or if bustools count
without --genecounts
is selected and then kallisto quant-tcc
is ran to estimate isoform abundances from TCC counts
@sbooeshaghi, I went through your notebook on the mouse retinal cell analysis. During the normalization of the data using
scv.pp.normalize_per_cell(adata)
, scVelo is throwing the warningWARNING: Did not normalize X as it looks processed already. To enforce normalization, set
enforce=True.
(same for layersunspliced
andspliced
). I had a look at the data and this is caused by the counts not being integers. Are the reads that kallisto bustools provides pseudo counts?