pachterlab / kallisto

Near-optimal RNA-Seq quantification
https://pachterlab.github.io/kallisto
BSD 2-Clause "Simplified" License
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feature request: output un-pseudoaligned reads #163

Open rschulzUK opened 6 years ago

rschulzUK commented 6 years ago

WIth typical kallisto pseudoalignment rates of ~70% for human total ribo-depleted RNA libraries, an obvious question is what the remaining 30% of reads represent, especially when aligners like hisat2 report alignment rates >97%. Given the speed advantage of kallisto, a reasonable workflow would be to only align the un-pseudoaligned reads afterwards to see what they are and quantify them separately with something like featureCounts. A kallisto option for dumping the fastq records of un-pseudoaligned reads into a file therefore would be very helpful. Cheers, r

arogozhnikov commented 5 years ago

I'd suggest to output per-read alignment information if requested. This will address this issue, and also will help with comparing alignments