Describe the issuekb info returns a non-zero exit status, which is unexpected since no errors occurred and no undefined syntax was used.
What is the exact command that was run?
kb info
echo $?
Command output (with --verbose flag)
> kb info --verbose
kb_python 0.27.3
kallisto: 0.48.0 (/tmp/tmp.889zeieDYs/env/lib/python3.10/site-packages/kb_python/bins/linux/kallisto/kallisto)
bustools: 0.41.0 (/tmp/tmp.889zeieDYs/env/lib/python3.10/site-packages/kb_python/bins/linux/bustools/bustools)
kb is a python package for rapidly pre-processing single-cell RNA-seq data. It
is a wrapper for the methods described in [2].
The goal of the wrapper is to simplify downloading and running of the kallisto
[1] and bustools [2] programs. It was inspired by Sten Linnarsson’s loompy
fromfq command (http://linnarssonlab.org/loompy/kallisto/index.html)
The kb program consists of two parts:
The `kb ref` command builds or downloads a species-specific index for
pseudoalignment of reads. This command must be run prior to `kb count`, and it
runs the `kallisto index` [1].
The `kb count` command runs the kallisto [1] and bustools [2] programs. It can
be used for pre-processing of data from a variety of single-cell RNA-seq
technologies, and for a number of different workflows (e.g. production of gene
count matrices, RNA velocity analyses, etc.). The output can be saved in a
variety of formats including mix and loom. Examples are provided below.
Examples are available at: https://www.kallistobus.tools/tutorials
References
==========
[1] Bray, N. L., Pimentel, H., Melsted, P., & Pachter, L. (2016). Near-optimal
probabilistic RNA-seq quantification. Nature biotechnology, 34(5), 525.
[2] Melsted, P., Booeshaghi, A. S., Liu, L., Gao, F., Lu, L., Min, K. H., da
Veiga Beltrame, E., Hjorleifsson, K. E., Gehring, J., & Pachter, L. (2021).
Modular and efficient pre-processing of single-cell RNA-seq. Nature
Biotechnology.
> echo $?
1
Describe the issue
kb info
returns a non-zero exit status, which is unexpected since no errors occurred and no undefined syntax was used.What is the exact command that was run?
Command output (with
--verbose
flag)