Hello.
I am now facing a problem when I library Sleuth in R. The command is so <- sleuth_fit(so, ~condition, 'full'), and the problem is (4 NA values were found during variance shrinkage estimation due to mean observation values outside of the range used for the LOESS fit.
The LOESS fit will be repeated using exact computation of the fitted surface to extrapolate the missing values.These are the target ids with NA values: PPYR_00001-PA, PPYR_03317-PA, PPYR_05506-PA, PPYR_11147-PA).
And I don't konw what's the reason and how to deal with it, Could you please help me?
Hello. I am now facing a problem when I library Sleuth in R. The command is so <- sleuth_fit(so, ~condition, 'full'), and the problem is (4 NA values were found during variance shrinkage estimation due to mean observation values outside of the range used for the LOESS fit. The LOESS fit will be repeated using exact computation of the fitted surface to extrapolate the missing values.These are the target ids with NA values: PPYR_00001-PA, PPYR_03317-PA, PPYR_05506-PA, PPYR_11147-PA). And I don't konw what's the reason and how to deal with it, Could you please help me?
best wishes!