I've been searching for an answer about which data frame to use after sleuth_prep step. After sleuth_prep, I got obs_norm_filt, obs_norm and obs_raw, each of them contains a tpm value. I don't understand what's the difference among those data frames. Since tpm contains normalized counts already, what is the tpm in obs_norm or obs_norm_filt dataset? what normalization it used? Thank you!
Hello,
I've been searching for an answer about which data frame to use after sleuth_prep step. After sleuth_prep, I got obs_norm_filt, obs_norm and obs_raw, each of them contains a tpm value. I don't understand what's the difference among those data frames. Since tpm contains normalized counts already, what is the tpm in obs_norm or obs_norm_filt dataset? what normalization it used? Thank you!